DOI: 10.1016/s0014-5793(00)01896-2. EGFP and DsRed expressing cultures of <i>Escherichia coli</i> imaged by confocal, two‐photon and fluorescence lifetime microscopy

EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two‐photon and fluorescence lifetime microscopy

10.1016/s0014-5793(00)01896-2

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The green fluorescent protein (GFP) has become an invaluable marker for monitoring protein localization and gene expression in vivo. Recently a new red fluorescent protein (drFP583 or DsRed), isolated from tropical corals, has been described [Matz, M.V. et al. (1999) Nature Biotech. 17, 969–973]. With emission maxima at 509 and 583 nm respectively, EGFP and DsRed are suited for almost crossover free dual color labeling upon simultaneous excitation. We imaged mixed populations of Escherichia coli expressing either EGFP or DsRed by one‐photon confocal and by two‐photon microscopy. Both excitation modes proved to be suitable for imaging cells expressing either of the fluorescent proteins. DsRed had an extended maturation time and E. coli expressing this fluorescent protein were significantly smaller than those expressing EGFP. In aging bacterial cultures DsRed appeared to aggregate within the cells, accompanied by a strong reduction in its fluorescence lifetime as determined by fluorescence lifetime imaging microscopy.

Bibliography

Jakobs, S., Subramaniam, V., SchÃ¶nle, A., Jovin, T. M., & Hell, S. W. (2000). EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, twoâphoton and fluorescence lifetime microscopy. FEBS Letters, 479(3), 131â135. Portico.

Authors 5

Stefan Jakobs (first)
Vinod Subramaniam (additional)
Andreas Schönle (additional)
Thomas M. Jovin (additional)
Stefan W. Hell (additional)

References 25 Referenced 149

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:18 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:09 a.m.)
Indexed	1 month, 3 weeks ago (July 12, 2025, 6:48 p.m.)
Issued	25 years ago (Aug. 14, 2000)
Published	25 years ago (Aug. 14, 2000)
Published Online	25 years ago (Aug. 14, 2000)
Published Print	25 years ago (Aug. 18, 2000)

Funders 0

None

BibTeX

@article{Jakobs_2000, title={EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two‐photon and fluorescence lifetime microscopy}, volume={479}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(00)01896-2}, DOI={10.1016/s0014-5793(00)01896-2}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Jakobs, Stefan and Subramaniam, Vinod and Schönle, Andreas and Jovin, Thomas M. and Hell, Stefan W.}, year={2000}, month=aug, pages={131–135} }

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