DOI: 10.1016/s0014-5793(00)01773-7. Functional genomics by mass spectrometry

Functional genomics by mass spectrometry

10.1016/s0014-5793(00)01773-7

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Systematic analysis of the function of genes can take place at the oligonucleotide or protein level. The latter has the advantage of being closest to function, since it is proteins that perform most of the reactions necessary for the cell. For most protein based (‘proteomic’) approaches to gene function, mass spectrometry is the method of choice. Mass spectrometry can now identify proteins with very high sensitivity and medium to high throughput. New instrumentation for the analysis of the proteome has been developed including a MALDI hybrid quadrupole time of flight instrument which combines advantages of the mass finger printing and peptide sequencing methods for protein identification. New approaches include the isotopic labeling of proteins to obtain accurate quantitative data by mass spectrometry, methods to analyze peptides derived from crude protein mixtures and approaches to analyze large numbers of intact proteins by mass spectrometry directly. Examples from this laboratory illustrate biological problem solving by modern mass spectrometric techniques. These include the analysis of the structure and function of the nucleolus and the analysis of signaling complexes.

Bibliography

Andersen, J. S., & Mann, M. (2000). Functional genomics by mass spectrometry. FEBS Letters, 480(1), 25â31. Portico.

Authors 2

Jens S Andersen (first)
Matthias Mann (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 12:23 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 9:35 p.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 9:14 p.m.)
Issued	25 years ago (Aug. 24, 2000)
Published	25 years ago (Aug. 24, 2000)
Published Online	25 years ago (Aug. 24, 2000)
Published Print	25 years ago (Aug. 25, 2000)

Funders 0

None

BibTeX

@article{Andersen_2000, title={Functional genomics by mass spectrometry}, volume={480}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(00)01773-7}, DOI={10.1016/s0014-5793(00)01773-7}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Andersen, Jens S and Mann, Matthias}, year={2000}, month=aug, pages={25–31} }

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