DOI: 10.1016/s0014-5793(00)01704-x. States and transitions during forced unfolding of a single spectrin repeat

States and transitions during forced unfolding of a single spectrin repeat

10.1016/s0014-5793(00)01704-x

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Spectrin is a vital and abundant protein of the cytoskeleton. It has an elongated structure that is made by a chain of so‐called spectrin repeats. Each repeat contains three antiparallel α‐helices that form a coiled‐coil structure. Spectrin forms an oligomeric structure that is able to cross‐link actin filaments. In red cells, the spectrin/actin meshwork underlying cell membrane is thought to be responsible for special elastic properties of the cell. In order to determine mechanical unfolding properties of the spectrin repeat, we have used single molecule force spectroscopy to study the states of unfolding of an engineered polymeric protein consisting of identical spectrin domains. We demonstrate that the unfolding of spectrin domains can occur in a stepwise fashion during stretching. The force–extension patterns exhibit features that are compatible with the existence of at least one intermediate between the folded and the completely unfolded conformation. Only those polypeptides that still contain multiple intact repeats display intermediates, indicating a stabilisation effect. Precise force spectroscopy measurements on single molecules using engineered protein constructs reveal states and transitions during the mechanical unfolding of spectrin. Single molecule force spectroscopy appears to open a new window for the analysis of transition probabilities between different conformational states.

Bibliography

Lenne, P.-F., Raae, A. J., Altmann, S. M., Saraste, M., & HÃ¶rber, J. K. H. (2000). States and transitions during forced unfolding of a single spectrin repeat. FEBS Letters, 476(3), 124â128. Portico.

Authors 5

P.-F Lenne (first)
A.J Raae (additional)
S.M Altmann (additional)
M Saraste (additional)
J.K.H Hörber (additional)

References 25 Referenced 106

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 9:59 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 9:19 p.m.)
Indexed	11 months, 4 weeks ago (Sept. 5, 2024, 7:08 a.m.)
Issued	25 years, 1 month ago (July 3, 2000)
Published	25 years, 1 month ago (July 3, 2000)
Published Online	25 years, 1 month ago (July 3, 2000)
Published Print	25 years, 1 month ago (July 7, 2000)

Funders 0

None

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