DOI: 10.1016/s0014-5793(00)01466-6. Translation termination factor aRF1 from the archaeon <i>Methanococcus jannaschii</i> is active with eukaryotic ribosomes

Translation termination factor aRF1 from the archaeon Methanococcus jannaschii is active with eukaryotic ribosomes

10.1016/s0014-5793(00)01466-6

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Class‐1 translation termination factors (release factors (RFs)) from Eukarya (eRF1) and Archaea (aRF1) exhibit a high degree of amino acid sequence homology and share many common motifs. In contrast to eRF1, function(s) of aRF1 have not yet been studied in vitro. Here, we describe for the first time the cloning and expression in Escherichia coli of the gene encoding the peptide chain RF from the hyperthermophilic archaeon Methanococcus jannaschii (MjaRF1). In an in vitro assay with mammalian ribosomes, MjaRF1, which was overproduced in E. coli, was active as a RF with all three termination codon‐containing tetraplets, demonstrating the functional resemblance of aRF1 and eRF1. This observation confirms the earlier prediction that eRF1 and aRF1 form a common structural–functional eRF1/aRF1 protein family, originating from a common ancient ancestor.

Bibliography

Dontsova, M., Frolova, L., Vassilieva, J., Piendl, W., Kisselev, L., & Garber, M. (2000). Translation termination factor aRF1 from the archaeon Methanococcus jannaschii is active with eukaryotic ribosomes. FEBS Letters, 472(2â3), 213â216. Portico.

Authors 6

Maria Dontsova (first)
Ludmila Frolova (additional)
Julia Vassilieva (additional)
Wolfgang Piendl (additional)
Lev Kisselev (additional)
Maria Garber (additional)

References 25 Referenced 32

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Dates

Type	When
Created	23 years ago (July 25, 2002, 10:30 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:35 p.m.)
Indexed	1 month, 1 week ago (July 11, 2025, 6:15 a.m.)
Issued	25 years, 3 months ago (April 27, 2000)
Published	25 years, 3 months ago (April 27, 2000)
Published Online	25 years, 3 months ago (April 27, 2000)
Published Print	25 years, 3 months ago (April 28, 2000)

Funders 0

None

BibTeX

@article{Dontsova_2000, title={Translation termination factor aRF1 from the archaeon Methanococcus jannaschii is active with eukaryotic ribosomes}, volume={472}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(00)01466-6}, DOI={10.1016/s0014-5793(00)01466-6}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Dontsova, Maria and Frolova, Ludmila and Vassilieva, Julia and Piendl, Wolfgang and Kisselev, Lev and Garber, Maria}, year={2000}, month=apr, pages={213–216} }

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