DOI: 10.1016/s0014-5793(00)01208-4. Two different modes of cyclin Clb2 proteolysis during mitosis in <i>Saccharomyces cerevisiae</i>

Two different modes of cyclin Clb2 proteolysis during mitosis in Saccharomyces cerevisiae

10.1016/s0014-5793(00)01208-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Sister chromatid separation and mitotic exit are triggered by the anaphase‐promoting complex (APC/C) which is a multi‐subunit ubiquitin ligase required for proteolytic degradation of various target proteins. Cdc20 and Cdh1 are substrate‐specific activators of the APC/C. It was previously proposed that Cdh1 is essential for proteolysis of the yeast mitotic cyclin Clb2. We show that Clb2 proteolysis is triggered by two different modes during mitosis. A fraction of Clb2 is degraded during anaphase in the absence of Cdh1. However, a second fraction of Clb2 remains stable during anaphase and is degraded in a Cdh1‐dependent manner as cells exit from mitosis. Most of cyclin Clb3 is degraded independently of Cdh1. Our data imply that degradation of mitotic cyclins is initiated by a Cdh1‐independent mechanism.

Bibliography

BÃ¤umer, M., Braus, G. H., & Irniger, S. (2000). Two different modes of cyclin Clb2 proteolysis during mitosis in Saccharomyces cerevisiae. FEBS Letters, 468(2â3), 142â148. Portico.

Authors 3

Matthias Bäumer (first)
Gerhard H Braus (additional)
Stefan Irniger (additional)

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 9:35 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:29 a.m.)
Indexed	5 months, 1 week ago (March 22, 2025, 4:29 a.m.)
Issued	25 years, 6 months ago (Feb. 22, 2000)
Published	25 years, 6 months ago (Feb. 22, 2000)
Published Online	25 years, 6 months ago (Feb. 22, 2000)
Published Print	25 years, 6 months ago (Feb. 25, 2000)

Funders 0

None

BibTeX

@article{B_umer_2000, title={Two different modes of cyclin Clb2 proteolysis during mitosis in Saccharomyces cerevisiae}, volume={468}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(00)01208-4}, DOI={10.1016/s0014-5793(00)01208-4}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Bäumer, Matthias and Braus, Gerhard H and Irniger, Stefan}, year={2000}, month=feb, pages={142–148} }

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