DOI: 10.1016/s0014-5793(00)01192-3. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the Down critical region

The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the Down critical region

10.1016/s0014-5793(00)01192-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

We applied cDNA selection methods to a genomic clone (YAC 761B5) from chromosome 21 located in the so‐called ‘Down critical region’ in 21q22.3. Starting from human fetal heart and brain mRNAs we obtained and sequenced several cDNA clones. One of these clones (Down region aspartic protease (DRAP), named also BACE2 according to the gene nomenclature) revealed a striking nucleotide and amino acid sequence identity with several motifs present in members of the aspartic protease family. In particular the amino acid sequences comprising the two catalytic sites found in all mammalian aspartic proteases are perfectly conserved. Interestingly, the predicted protein shows a typical membrane spanning region; this is at variance with most other known aspartic proteases, which are soluble molecules. We present preliminary evidence, on the basis of in vitro translation studies and cell transfection, that this gene encodes a glycosylated protein which localizes mainly intracellularly but to some extent also to the plasma membrane. Furthermore DRAP/BACE2 shares a high homology with a newly described β‐secretase enzyme (BACE‐1) which is a transmembrane aspartic protease. The implications of this finding for Down syndrome are discussed.

Bibliography

Acquati, F., Accarino, M., Nucci, C., Fumagalli, P., Jovine, L., Ottolenghi, S., & Taramelli, R. (2000). The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the Down critical region. FEBS Letters, 468(1), 59â64. Portico.

Authors 7

F. Acquati (first)
M. Accarino (additional)
C. Nucci (additional)
P. Fumagalli (additional)
L. Jovine (additional)
S. Ottolenghi (additional)
R. Taramelli (additional)

References 34 Referenced 94

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 1:52 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 4:41 p.m.)
Indexed	2 days, 12 hours ago (Sept. 3, 2025, 6:56 a.m.)
Issued	25 years, 6 months ago (Feb. 14, 2000)
Published	25 years, 6 months ago (Feb. 14, 2000)
Published Online	25 years, 6 months ago (Feb. 14, 2000)
Published Print	25 years, 6 months ago (Feb. 18, 2000)

Funders 0

None

BibTeX

@article{Acquati_2000, title={The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the Down critical region}, volume={468}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/s0014-5793(00)01192-3}, DOI={10.1016/s0014-5793(00)01192-3}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Acquati, F. and Accarino, M. and Nucci, C. and Fumagalli, P. and Jovine, L. and Ottolenghi, S. and Taramelli, R.}, year={2000}, month=feb, pages={59–64} }

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