DOI: 10.1016/0378-1119(85)90140-4. Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites

10.1016/0378-1119(85)90140-4

Crossref journal-article

Elsevier BV

Gene (78)

Bibliography

Wells, J. A., Vasser, M., & Powers, D. B. (1985). Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites. Gene, 34(2â3), 315â323.

Authors 3

James A. Wells (first)
Mark Vasser (additional)
David B. Powers (additional)

References 26 Referenced 219

10.1089/dna.1.1984.3.17 / DNA / Bacillus subtilis requires a “stringent” Shine-Dalgarno region for gene expression by Band (1984)
10.1093/nar/7.6.1513 / Nucl. Acids Res. / A rapid alkaline extraction procedure for screening recombinant plasmid DNA by Birnboim (1979)
10.1089/dna.1985.4.165 / DNA / Supercoil sequencing: a fast and simple method for sequencing plasmid DNA by Chen (1985)
10.1093/nar/8.10.2331 / Nucl. Acids Res. / Synthesis of oligonucleotides on cellulose by a phosphotriester method by Crea (1980)
10.1111/j.1432-1033.1972.tb01754.x / Eur. J. Biochem. / Subtilisin Novo, the three-dimensional structure and its comparison with subtilisin BPN' by Drenth (1972)
{'key': '10.1016/0378-1119(85)90140-4_BIB6', 'first-page': '6409', 'article-title': 'Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function', 'volume': '79', 'author': 'Dalbadie-McFarland', 'year': '1982'} / Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function by Dalbadie-McFarland (1982)
10.1002/j.1460-2075.1984.tb01858.x / EMBO J. / Mutagenesis of the three bases preceding the start codon of the β-galactosidase mRNA and its effect on translation in Escherichia coli by Hui (1984)
10.1016/S0021-9258(19)46967-6 / J. Biol. Chem. / Mutagenesis of a specifie position in a DNA sequence by Hutchinson (1978)
10.1016/0022-2836(70)90051-3 / J.Mol. Biol. / Calcium-dependent bacteriophage DNA infection by Mandel (1970)
{'key': '10.1016/0378-1119(85)90140-4_BIB10', 'author': 'Maniatis', 'year': '1982'} by Maniatis (1982)
10.1093/nar/11.10.3113 / Nucl. Acids Res. / Targeted random mutagenesis: the use of ambiguously synthesized oligonucleotides to mutagenize sequences immediately 5′ of an ATG initiation codon by Matteucci (1983)
10.1016/0378-1119(82)90016-6 / Gene / A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments by Messing (1982)
10.1093/nar/9.2.309 / Nucl. Acids Res. / A system for shotgun DNA sequencing by Messing (1981)
{'key': '10.1016/0378-1119(85)90140-4_BIB14', 'series-title': 'Experiments in Molecular Genetics', 'author': 'Miller', 'year': '1972'} / Experiments in Molecular Genetics by Miller (1972)
10.1093/nar/11.15.5103 / Nucl. Acids Res. / Efficient site-directed mutagenesis by simultaneous use of two primers by Norris (1983)
10.1093/nar/11.24.8595 / Nucl. Acids Res. / In vitro site-directed mutagenesis with synthetic DNA oligonucleotides yields unexpected deletions and insertions at high frequency by Osinga (1983)
{'key': '10.1016/0378-1119(85)90140-4_BIB17', 'first-page': '4268', 'article-title': 'Efficient correction of a mutation by use of chemically synthesized DNA', 'volume': '75', 'author': 'Razin', 'year': '1978'} / Efficient correction of a mutation by use of chemically synthesized DNA by Razin (1978)
10.1016/0022-2836(80)90196-5 / J. Mol. Biol. / Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing by Sanger (1980)
10.1016/S0021-9258(18)63664-6 / J. Biol. Chem. / The effect on subtilisin activity of oxidizing a methionine residue by Stauffer (1969)
10.1126/science.6356360 / Science / Directed mutagenesis of dihydrofolate reductase by Villafranca (1983)
10.1093/nar/9.15.3647 / Nucleic Acids Res. / Oligonucleotide-directed mutagenesis of the human β-globin gene: a general method for producing specific point mutations in cloned DNA by Wallace (1981)
10.1093/nar/11.22.7911 / Nucl. Acids Res. / Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis by Wells (1983)
10.1038/299756a0 / Nature / Redesigning enzyme structure by site-directed mutagenesis: tyrosyl tRNA synthetase and ATP binding by Winter (1982)
10.1038/221235a0 / Nature / Structure of subtilisin BPN' at 25Å resolution by Wright (1969)
10.1128/JB.160.1.15-21.1984 / J. Bacteriol. / Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to generate an in vitro deletion mutation by Yang (1984)
10.1093/nar/10.20.6487 / Nucl. Acids Res. / Oligonucleotide-directed mutagenesis using M-13 derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA by Zoller (1982)

Dates

Type	When
Created	22 years, 6 months ago (Feb. 11, 2003, 10:07 p.m.)
Deposited	4 years, 2 months ago (June 4, 2021, 3:18 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 2, 2025, 1:16 a.m.)
Issued	40 years, 7 months ago (Jan. 1, 1985)
Published	40 years, 7 months ago (Jan. 1, 1985)
Published Print	40 years, 7 months ago (Jan. 1, 1985)

Funders 0

None

BibTeX

@article{Wells_1985, title={Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites}, volume={34}, ISSN={0378-1119}, url={http://dx.doi.org/10.1016/0378-1119(85)90140-4}, DOI={10.1016/0378-1119(85)90140-4}, number={2–3}, journal={Gene}, publisher={Elsevier BV}, author={Wells, James A. and Vasser, Mark and Powers, David B.}, year={1985}, month=jan, pages={315–323} }

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