DOI: 10.1016/0014-5793(96)01097-6. Phosphorylation of the eIF4E‐binding protein PHAS‐I after exposure of PC12 cells to EGF and NGF

Phosphorylation of the eIF4E‐binding protein PHAS‐I after exposure of PC12 cells to EGF and NGF

10.1016/0014-5793(96)01097-6

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

PHAS‐I or the eIF4E‐binding protein 1 regulates the cap‐binding activity of eIF4E by sequestering eIF4E. Binding of eIF4E to PHAS‐I is regulated by phosphorylation of PHAS‐I. PC12 cells were used to study the signal transduction pathway leading to phosphorylation of PHAS‐I. Both EGF and NGF induced phosphorylation of PHAS‐I. Wortmannin, a PI‐3 kinase inhibitor, staurosporine, a PKC inhibitor, and rapamycin, a FRAP inhibitor all blocked the phosphorylation of PHAS‐I. Of the three inhibitors, only wortmannin was able to inhibit MAPK phosphorylation. This excludes a role for MAPK in NGF‐ and EGF‐induced PHAS‐I phosphorylation in PC12 cells. Apparently, PHAS‐I was phosphorylated in a PI‐3 kinase‐, PKC‐, and FRAP‐dependent manner after EGF or NGF stimulation. Only PI‐3 kinase and FRAP are involved in the regulation of the basal level of PHAS‐I phosphorylation.

Bibliography

Kleijn, M., Korthout, M. M. R., Voorma, H. O., & Thomas, A. A. M. (1996). Phosphorylation of the eIF4Eâbinding protein PHASâI after exposure of PC12 cells to EGF and NGF. FEBS Letters, 396(2â3), 165â171. Portico.

Authors 4

Miranda Kleijn (first)
Marsha M.R. Korthout (additional)
Harry O. Voorma (additional)
Adri A.M. Thomas (additional)

References 31 Referenced 17

1994 W.B. Minich M.L. Balasta D.J. Goss R.E. Rhoads Proc. Natl. Acad. Sci. USA 7668 7672
10.1016/S0021-9258(19)39897-7
10.1016/S0021-9258(19)39358-5
1994 C. Hu S. Pang X. Kong M. Velleca J.C. Lawrence Proc. Natl. Acad. Sci. USA 3730 3734
10.1038/371762a0
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10.1074/jbc.270.37.21975
10.1002/j.1460-2075.1995.tb00257.x
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10.1042/bj2140011
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1996 S.R. Von Manteuffel A.-C. Gringras X.-F. Ming N. Sonenberg G. Thomas Proc. Natl. Acad. Sci. USA 4076 4080

Dates

Type	When
Created	22 years, 4 months ago (April 24, 2003, 9:18 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 1:33 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:45 p.m.)
Issued	28 years, 9 months ago (Nov. 4, 1996)
Published	28 years, 9 months ago (Nov. 4, 1996)
Published Online	25 years, 8 months ago (Dec. 2, 1999)
Published Print	28 years, 9 months ago (Nov. 4, 1996)

Funders 0

None

BibTeX

@article{Kleijn_1996, title={Phosphorylation of the eIF4E‐binding protein PHAS‐I after exposure of PC12 cells to EGF and NGF}, volume={396}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(96)01097-6}, DOI={10.1016/0014-5793(96)01097-6}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Kleijn, Miranda and Korthout, Marsha M.R. and Voorma, Harry O. and Thomas, Adri A.M.}, year={1996}, month=nov, pages={165–171} }

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