DOI: 10.1016/0014-5793(96)01042-3. Multiple inhibitory effects of Alzheimer's peptide Aβ1–40 on lipid biosynthesis in cultured human HepG2 cells

Multiple inhibitory effects of Alzheimer's peptide Aβ1–40 on lipid biosynthesis in cultured human HepG2 cells

10.1016/0014-5793(96)01042-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Herein we describe the inhibitory effect of the synthetic peptide Aβ1–40, homologous to the major high‐density lipoprotein‐associated species of Alzheimer's amyloid β protein (Aβ), on lipid biosynthesis in human hepatic HepG2 cells. This culture synthesizes various lipids from [14C]acetate as a precursor. Treatment of cells with different concentrations of Aβ1–40 decreased the syntheses of various radiolabeled lipid species. The decrease reached saturation at peptide concentrations equal to 10–100 ng ml−1. The lipids whose synthesis was decreased most were free and esterified cholesterol and phospholipids. This inhibitory effect suggests that Aβ protein may modulate physiological intracellular lipid syntheses. It may also be of special importance in the pathological condition, and contribute to the neurodegeneration, in Alzheimer's disease and related disorders.

Bibliography

Koudinova, N. V., Berezov, T. T., & Koudinov, A. R. (1996). Multiple inhibitory effects of Alzheimerâs peptide AÎ²1â40 on lipid biosynthesis in cultured human HepG2 cells. FEBS Letters, 395(2â3), 204â206. Portico.

Authors 3

Natalia V Koudinova (first)
Temirbolat T Berezov (additional)
Alexei R Koudinov (additional)

References 30 Referenced 23

10.1016/0166-2236(93)90008-A
1989 D Rosen L Martin-Morris L Luo K White Proc. Natl. Acad. Sci. USA 2478 2482
10.1016/0197-4580(94)90115-5
{'key': 'e_1_2_1_5_1', 'first-page': '1423', 'volume': '138', 'author': 'Podlisny M', 'year': '1991', 'journal-title': 'Am. J. Pathol.'} / Am. J. Pathol. by Podlisny M (1991)
10.1006/bbrc.1994.2788
1995 New York University Medical Center New York A Koudinov N Koudinova A Kumar Neuroscience Meeting Abstract Book
10.1006/bbrc.1996.0940
1994 Piter St. Petersburg A Klimov N Nikulcheva Lipids Lipoproteins and Atherosclerosis 95 153
10.1016/S0022-2275(20)39898-9
10.1016/S0022-2275(20)34443-6
{'key': 'e_1_2_1_12_1', 'first-page': '747', 'volume': '38', 'author': 'Koudinov A', 'year': '1996', 'journal-title': 'Biochem. Mol. Biol. Int.'} / Biochem. Mol. Biol. Int. by Koudinov A (1996)
{'key': 'e_1_2_1_13_1', 'first-page': '489', 'volume': '128', 'author': 'Marsh J', 'year': '1986', 'journal-title': 'Methods Enzymol.'} / Methods Enzymol. by Marsh J (1986)
1992 Mir Moscow T Berezov B Korovkin Biochemistry 257 294
10.1096/fasebj.4.2.2153592
1993 Oxford University Press New York P Monaghan D Robertson J Beesley J Beesley Immunocyto-chemistry. A Practical Approach 4 76
1966 B Rotman B Papaermaster Proc. Natl. Acad. Sci. USA 134 137
10.1016/0300-483X(88)90132-1
10.1016/0003-2697(78)90046-5
{'key': 'e_1_2_1_20_1', 'first-page': '442', 'volume': '14', 'author': 'Skipski V', 'year': '1969', 'journal-title': 'Methods Enzymol.'} / Methods Enzymol. by Skipski V (1969)
N Koudinova 1992 Russian State Medical University Moscow 34
1990 Pirogov Second Medical Institute Moscow E Zanina Methods of Statistical Analysis Handbook
10.1016/0005-2760(88)90082-3
10.1007/BF03160167
10.1016/0006-8993(93)90050-W
1988 Dekker New York J Pettegrew W Klunk R McClure K Panchalingam S Strychor Z Khachuturian J Blass Alzheimer's Disease. New Treatment Strategies 200 205
10.1016/0197-4580(92)90116-F
10.1006/exnr.1994.1044
10.1007/BF02191587
10.1212/WNL.45.6.1092
1992 R Nitsch J Blusztajn A Pittas B Slack J Growdon R Wurtman Proc. Natl. Acad. Sci. USA 1671 1675

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 12:08 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 3:35 a.m.)
Indexed	2 months, 3 weeks ago (June 12, 2025, 5:43 a.m.)
Issued	28 years, 10 months ago (Oct. 21, 1996)
Published	28 years, 10 months ago (Oct. 21, 1996)
Published Online	22 years ago (Aug. 5, 2003)
Published Print	28 years, 10 months ago (Oct. 21, 1996)

Funders 0

None

BibTeX

@article{Koudinova_1996, title={Multiple inhibitory effects of Alzheimer’s peptide Aβ1–40 on lipid biosynthesis in cultured human HepG2 cells}, volume={395}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(96)01042-3}, DOI={10.1016/0014-5793(96)01042-3}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Koudinova, Natalia V and Berezov, Temirbolat T and Koudinov, Alexei R}, year={1996}, month=oct, pages={204–206} }

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