DOI: 10.1016/0014-5793(96)00873-3. Dominant role of mitochondria in protection against a delayed neuronal Ca<sup>2+</sup> overload induced by endogenous excitatory amino acids following a glutamate pulse

Dominant role of mitochondria in protection against a delayed neuronal Ca2+ overload induced by endogenous excitatory amino acids following a glutamate pulse

10.1016/0014-5793(96)00873-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The objective of this study was to evaluate the contribution of mitochondria to the clearance of Ca2+ loads induced by glutamate or 25 mM K+ pulses. The mitochondrial Ca2+ uptake was suppressed by application of 0.5 μM antimycin A or 3–5 mM NaCN in combination with 2.5 μg/ml oligomycin. In most cells such treatments both in the presence and in the absence of external Na+ failed to abolish the early fast phase of [Ca2+] i recovery following a 1‐min 100 μM glutamate pulse. However, the late slow phase of [Ca2+] i recovery in the presence of mitochondrial poisons was transformed into a delayed [Ca2+] i elevation culminating in the neuronal Ca2+ overload. Suppression of the Na+/Ca2+ exchange caused by glutamate‐induced [Na+] i elevation promoted the development of delayed Ca2+ increase. Under identical conditions, the high [Ca2+] i transient induced by 25 mM K+ was never accompanied by a delayed Ca2+ elevation. The glutamate‐induced delayed Ca2+ increase could be readily abolished by the removal of external Ca2+ or by application in the post‐glutamate period of the antagonist of NMDA receptors, 100–200 μM AP‐5. The results obtained suggest that mitochondria play a dominant role in the protection against the neuronal Ca2+ overload induced by endogenous excitatory amino acids released in response to a short‐term glutamate challenge.

Bibliography

Khodorov, B., Pinelis, V., Storozhevykh, T., Vergun, O., & Vinskaya, N. (1996). Dominant role of mitochondria in protection against a delayed neuronal Ca2+ overload induced by endogenous excitatory amino acids following a glutamate pulse. FEBS Letters, 393(1), 135â138. Portico.

Authors 5

B. Khodorov (first)
V. Pinelis (additional)
T. Storozhevykh (additional)
O. Vergun (additional)
N. Vinskaya (additional)

References 13 Referenced 51

10.1523/JNEUROSCI.15-02-01318.1995
10.1046/j.1471-4159.1996.66010403.x
10.1016/0006-8993(91)91141-M
10.1016/0014-5793(95)00894-F
10.1016/0896-6273(94)90272-0
{'key': 'e_1_2_1_7_1', 'first-page': '475', 'volume': '32', 'author': 'Pinelis V.', 'year': '1994', 'journal-title': 'Biochem. Mol. Biol. Int.'} / Biochem. Mol. Biol. Int. by Pinelis V. (1994)
{'key': 'e_1_2_1_8_1', 'first-page': '285', 'volume-title': 'Proc. R. Soc. Lond.', 'author': 'Schwiening C.', 'year': '1993'} / Proc. R. Soc. Lond. by Schwiening C. (1993)
10.1007/BF00964817
10.1016/0304-3940(93)90679-F
10.1016/0306-4522(87)90347-2
{'key': 'e_1_2_1_12_1', 'first-page': '752', 'volume': '250', 'author': 'Hartley D.', 'year': '1989', 'journal-title': 'J. Pharmacol. Exp. Ther.'} / J. Pharmacol. Exp. Ther. by Hartley D. (1989)
10.1016/0165-6147(90)90129-V
10.1523/JNEUROSCI.13-05-02085.1993

Dates

Type	When
Created	22 years, 4 months ago (April 30, 2003, 6:24 p.m.)
Deposited	1 year, 11 months ago (Sept. 17, 2023, 3:04 a.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 1:24 a.m.)
Issued	28 years, 11 months ago (Sept. 9, 1996)
Published	28 years, 11 months ago (Sept. 9, 1996)
Published Online	26 years, 5 months ago (March 23, 1999)
Published Print	28 years, 11 months ago (Sept. 9, 1996)

Funders 0

None

BibTeX

@article{Khodorov_1996, title={Dominant role of mitochondria in protection against a delayed neuronal Ca2+ overload induced by endogenous excitatory amino acids following a glutamate pulse}, volume={393}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(96)00873-3}, DOI={10.1016/0014-5793(96)00873-3}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Khodorov, B. and Pinelis, V. and Storozhevykh, T. and Vergun, O. and Vinskaya, N.}, year={1996}, month=sep, pages={135–138} }

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