DOI: 10.1016/0014-5793(96)00277-3. Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity

Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity

10.1016/0014-5793(96)00277-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Using the differential display PCR method, we have isolated an mRNA downregulated in doxorubicin resistant human cell lines. The full length cDNA clone was identified as the human homologue of yeast RPB11 subunit of RNA polymerase II. Northern blot analysis of normal tissues detected a particularly high expression of RPB11 mRNA in heart and skeletal muscle. Reduction of this mRNA expression was observed in all the cell lines tested after drug treatment and was paralleled by a similar decrease of the protein levels. These findings suggest that doxorubicin may exert in vivo specific inhibitory effects on a major component of the transcription machinery.

Bibliography

Fanciulli, M., Bruno, T., Cerboni, C., Bonetto, F., Iacobini, C., Frati, L., Piccoli, M., Floridi, A., Santoni, A., & Punturieri, A. (1996). Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity. FEBS Letters, 384(1), 48â52. Portico.

Authors 10

Maurizio Fanciulli (first)
Tiziana Bruno (additional)
Cristina Cerboni (additional)
Francesco Bonetto (additional)
Carla Iacobini (additional)
Luigi Frati (additional)
Mario Piccoli (additional)
Aristide Floridi (additional)
Angela Santoni (additional)
Antonello Punturieri (additional)

References 22 Referenced 21

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Dates

Type	When
Created	22 years, 4 months ago (April 23, 2003, 4:36 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 13, 2024, 12:40 p.m.)
Issued	29 years, 4 months ago (April 8, 1996)
Published	29 years, 4 months ago (April 8, 1996)
Published Online	25 years, 9 months ago (Nov. 30, 1999)
Published Print	29 years, 4 months ago (April 8, 1996)

Funders 0

None

BibTeX

@article{Fanciulli_1996, title={Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity}, volume={384}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(96)00277-3}, DOI={10.1016/0014-5793(96)00277-3}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Fanciulli, Maurizio and Bruno, Tiziana and Cerboni, Cristina and Bonetto, Francesco and Iacobini, Carla and Frati, Luigi and Piccoli, Mario and Floridi, Aristide and Santoni, Angela and Punturieri, Antonello}, year={1996}, month=apr, pages={48–52} }

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