DOI: 10.1016/0014-5793(96)00101-9. Molecular cloning and expression of a multiubiquitin chain binding subunit of the human 26S protease

Molecular cloning and expression of a multiubiquitin chain binding subunit of the human 26S protease

10.1016/0014-5793(96)00101-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

S5a is a subunit of the 26S protease that binds and presumably selects multiubiquitinated proteins for destruction. We recently identified an Arabidopsis protein, MBP1, that is physically, immunologically and biochemically similar to S5a from the human erythrocyte 26S protease. Based upon the MBP1 cDNA sequence we have now isolated a HeLa cell cDNA coding for human S5a. The HeLa cDNA sequence is highly similar to MBP1 and it encodes peptides obtained directly from human erythrocyte S5a. Moreover, expression of the isolated cDNA in E. coli results in a recombinant protein with an apparent molecular mass and multiubiquitin binding properties that match those of human S5a obtained from the purified 26S enzyme.

Bibliography

Ferrell, K., Deveraux, Q., van Nocker, S., & Rechsteiner, M. (1996). Molecular cloning and expression of a multiubiquitin chain binding subunit of the human 26S protease. FEBS Letters, 381(1â2), 143â148. Portico.

Authors 4

Katherine Ferrell (first)
Quinn Deveraux (additional)
Steven van Nocker (additional)
Martin Rechsteiner (additional)

References 31 Referenced 61

10.1038/349132a0
10.1016/S0021-9258(18)55308-4
10.1101/gad.9.13.1586
{'key': 'e_1_2_1_5_1', 'first-page': '359', 'volume-title': 'Proc. Natl. Acad. Sci. USA', 'author': 'Shanklin J.', 'year': '1987'} / Proc. Natl. Acad. Sci. USA by Shanklin J. (1987)
10.1146/annurev.bi.61.070192.003553
10.1016/0092-8674(94)90396-4
10.1016/S0021-9258(18)53218-X
{'key': 'e_1_2_1_9_1', 'first-page': '187', 'volume': '121', 'author': 'Orlowski M.', 'year': '1993', 'journal-title': 'J. Lab. Clin. Med.'} / J. Lab. Clin. Med. by Orlowski M. (1993)
10.1042/bj2910001
10.1016/S0021-9258(18)41680-8
10.1016/S0021-9258(18)52977-X
10.1016/S0021-9258(17)37345-3
10.1016/S0021-9258(17)31904-X
10.1016/S0021-9258(18)37771-8
10.1007/BF00990967
10.1007/BF00990966
10.1016/0014-5793(95)00534-G
10.1016/0092-8674(89)90844-1
10.1074/jbc.270.40.23726
10.1016/0014-5793(95)00288-K
10.1002/j.1460-2075.1995.tb07313.x
10.1016/S0021-9258(17)37244-7
S.van Nocker Q.Deveraux M.RechsteinerandR.Vierstra (1996) PNAS in press.
F.M. Ausubel R. Brent R.E. Kingston D.D. Moore J.G. Seidman J.A. Smith K. Struhl 1992 Green Publishing Associates Wiley-Interscience New York
10.1016/0092-8674(86)90762-2
10.1016/0014-5793(94)00569-9
10.1111/j.1432-1033.1995.0720d.x
10.1074/jbc.270.35.20615
{'key': 'e_1_2_1_30_1', 'first-page': '139', 'volume': '883', 'author': 'Lönnroth I.', 'year': '1986', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta by Lönnroth I. (1986)
10.1074/jbc.270.40.23726
{'key': 'e_1_2_1_32_1', 'volume-title': 'Proc. Natl. Acad. Sci. USA', 'author': 'Beal R.', 'year': '1996'} / Proc. Natl. Acad. Sci. USA by Beal R. (1996)

Dates

Type	When
Created	22 years, 4 months ago (April 4, 2003, 7:21 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 8:40 a.m.)
Indexed	1 month, 2 weeks ago (July 11, 2025, 6:52 a.m.)
Issued	29 years, 6 months ago (Feb. 26, 1996)
Published	29 years, 6 months ago (Feb. 26, 1996)
Published Online	25 years, 8 months ago (Nov. 30, 1999)
Published Print	29 years, 6 months ago (Feb. 26, 1996)

Funders 0

None

BibTeX

@article{Ferrell_1996, title={Molecular cloning and expression of a multiubiquitin chain binding subunit of the human 26S protease}, volume={381}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(96)00101-9}, DOI={10.1016/0014-5793(96)00101-9}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Ferrell, Katherine and Deveraux, Quinn and van Nocker, Steven and Rechsteiner, Martin}, year={1996}, month=feb, pages={143–148} }

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