DOI: 10.1016/0014-5793(95)00487-t. ATP synthesis catalyzed by the mitochondrial F<sub>1</sub>‐F<sub>0</sub> ATP synthase is not a reversal of its ATPase activity

ATP synthesis catalyzed by the mitochondrial F1‐F0 ATP synthase is not a reversal of its ATPase activity

10.1016/0014-5793(95)00487-t

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The ADP(Mg2+)‐deactivated oligomycin‐sensitive F1‐F0 ATPase of coupled submitochondrial particles treated with the substoichiometric amount of oligomycin was studied to test whether ATP synthesis and hydrolysis proceed in either direction through the same intermediates. The initial rates of ATP hydrolysis, oxidative phosphorylation, ATP‐dependent, succinate‐supported NAD+ reduction, and ATP‐induced generation were measured using deactivated ATPase trapped by azide [Biochem. J. (1982) 202, 15–23]. Three ATP consuming reactions were strongly inhibited when azide was present in the assay mixtures, whereas ATP synthesis was not altered by azide. The unidirectional effect of azide is not consistent with three alternating binding sites mechanism operating in ATP synthesis and support our hypothesis on the existence of nucleotide(Mg2+)‐controlled ‘synthase’ and ‘hydrolase’ states of the mitochondrial F1‐F0 ATPase.

Bibliography

Syroeshkin, A. V., Vasilyeva, E. A., & Vinogradov, A. D. (1995). ATP synthesis catalyzed by the mitochondrial F1âF0 ATP synthase is not a reversal of its ATPase activity. FEBS Letters, 366(1), 29â32. Portico.

Authors 3

A.V. Syroeshkin (first)
E.A. Vasilyeva (additional)
A.D. Vinogradov (additional)

References 41 Referenced 47

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Dates

Type	When
Created	23 years ago (July 25, 2002, 9:47 a.m.)
Deposited	1 year, 11 months ago (Sept. 17, 2023, 2:37 a.m.)
Indexed	1 month, 3 weeks ago (June 27, 2025, 6:13 a.m.)
Issued	30 years, 2 months ago (June 5, 1995)
Published	30 years, 2 months ago (June 5, 1995)
Published Online	25 years, 5 months ago (March 7, 2000)
Published Print	30 years, 2 months ago (June 5, 1995)

Funders 0

None

BibTeX

@article{Syroeshkin_1995, title={ATP synthesis catalyzed by the mitochondrial F1‐F0 ATP synthase is not a reversal of its ATPase activity}, volume={366}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(95)00487-t}, DOI={10.1016/0014-5793(95)00487-t}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Syroeshkin, A.V. and Vasilyeva, E.A. and Vinogradov, A.D.}, year={1995}, month=jun, pages={29–32} }

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