DOI: 10.1016/0014-5793(95)00223-v. The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum

The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum

10.1016/0014-5793(95)00223-v

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Cross‐linking studies have implicated Sec61α as the principal component adjacent to newly synthesised membrane proteins during insertion into the endoplasmic reticulum. Using proteoliposomes which have been reconstituted from purified components of the endoplasmic reticulum [Görlich, D and Rapoport, T.A., Cell 75 (1993) 615–630] we have found that the Sec61 complex, consisting of three subunits, is essential for the insertion of single‐spanning membrane proteins. This is true for signal‐anchor proteins of both orientations, and for proteins with a cleavable signal sequence. These results support the view that Sec61α is a major component of the ER translocation site and promotes both the insertion of membrane proteins and the translocation of secretory proteins.

Bibliography

Oliver, J., Jungnickel, B., GÃ¶rlich, D., Rapoport, T., & High, S. (1995). The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum. FEBS Letters, 362(2), 126â130. Portico.

Authors 5

Jason Oliver (first)
Berit Jungnickel (additional)
Dirk Görlich (additional)
Tom Rapoport (additional)
Stephen High (additional)

References 32 Referenced 47

10.1002/bies.950140807
10.1083/jcb.108.4.1227
{'key': 'e_1_2_1_4_1', 'first-page': '5786', 'volume-title': 'Proc. Natl. Acad. Sci. USA', 'author': 'Hartmann E.', 'year': '1989'} / Proc. Natl. Acad. Sci. USA by Hartmann E. (1989)
10.1016/0955-0674(92)90075-N
10.1083/jcb.113.1.35
10.1146/annurev.cb.10.110194.000511
10.1083/jcb.121.4.743
10.1083/jcb.114.1.21
10.1083/jcb.112.5.809
10.1083/jcb.105.2.633
10.1016/0092-8674(92)90414-8
10.1016/0092-8674(92)90415-9
10.1016/0092-8674(92)90517-G
10.1038/357047a0
10.1016/S0021-9258(19)74376-2
10.1002/j.1460-2075.1994.tb06713.x
10.1083/jcb.116.3.597
10.1091/mbc.3.2.129
10.1038/367654a0
10.1016/0092-8674(93)90483-7
10.1002/j.1460-2075.1984.tb02271.x
10.1016/S0076-6879(83)96010-X
10.1016/S0076-6879(83)96057-3
10.1083/jcb.108.3.789
10.1083/jcb.113.1.25
10.1111/j.1432-1033.1982.tb06650.x
10.1042/bj2430277
10.1083/jcb.102.6.2169
{'key': 'e_1_2_1_30_1', 'first-page': '155', 'volume': '55', 'author': 'Gething M.-J.', 'year': '1989', 'journal-title': 'Biochem. Soc. Symp.'} / Biochem. Soc. Symp. by Gething M.-J. (1989)
10.1042/bj2540743
10.1002/j.1460-2075.1994.tb06788.x
10.1016/0014-5793(94)00367-X

Dates

Type	When
Created	22 years, 3 months ago (April 30, 2003, 6:24 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 1:55 a.m.)
Indexed	1 day, 15 hours ago (Aug. 21, 2025, 2:14 p.m.)
Issued	30 years, 4 months ago (April 3, 1995)
Published	30 years, 4 months ago (April 3, 1995)
Published Online	25 years, 5 months ago (March 7, 2000)
Published Print	30 years, 4 months ago (April 3, 1995)

Funders 0

None

BibTeX

@article{Oliver_1995, title={The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum}, volume={362}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(95)00223-v}, DOI={10.1016/0014-5793(95)00223-v}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Oliver, Jason and Jungnickel, Berit and Görlich, Dirk and Rapoport, Tom and High, Stephen}, year={1995}, month=apr, pages={126–130} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        21
      ]
    ],
    "date-time": "2025-08-21T18:14:05Z",
    "timestamp": 1755800045602
  },
  "reference-count": 32,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2000,
            3,
            7
          ]
        ],
        "date-time": "2000-03-07T00:00:00Z",
        "timestamp": 952387200000
      },
      "content-version": "vor",
      "delay-in-days": 1800,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        4,
        3
      ]
    ]
  },
  "abstract": "<jats:p>Cross\u2010linking studies have implicated Sec61\u03b1 as the principal component adjacent to newly synthesised membrane proteins during insertion into the endoplasmic reticulum. Using proteoliposomes which have been reconstituted from purified components of the endoplasmic reticulum [G\u00f6rlich, D and Rapoport, T.A., Cell 75 (1993) 615\u2013630] we have found that the Sec61 complex, consisting of three subunits, is essential for the insertion of single\u2010spanning membrane proteins. This is true for signal\u2010anchor proteins of both orientations, and for proteins with a cleavable signal sequence. These results support the view that Sec61\u03b1 is a major component of the ER translocation site and promotes both the insertion of membrane proteins and the translocation of secretory proteins.</jats:p>",
  "DOI": "10.1016/0014-5793(95)00223-v",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        4,
        30
      ]
    ],
    "date-time": "2003-04-30T22:24:07Z",
    "timestamp": 1051741447000
  },
  "page": "126-130",
  "source": "Crossref",
  "is-referenced-by-count": 47,
  "title": "The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum",
  "prefix": "10.1002",
  "volume": "362",
  "author": [
    {
      "given": "Jason",
      "family": "Oliver",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Berit",
      "family": "Jungnickel",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Dirk",
      "family": "G\u00f6rlich",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Tom",
      "family": "Rapoport",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Stephen",
      "family": "High",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2000,
        3,
        7
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bies.950140807"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.108.4.1227"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "5786",
      "volume-title": "Proc. Natl. Acad. Sci. USA",
      "author": "Hartmann E.",
      "year": "1989"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0955-0674(92)90075-N"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.113.1.35"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.cb.10.110194.000511"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.121.4.743"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.114.1.21"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.112.5.809"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.105.2.633"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90414-8"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90415-9"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90517-G"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/357047a0"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)74376-2"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1994.tb06713.x"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.116.3.597"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.3.2.129"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/367654a0"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(93)90483-7"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1984.tb02271.x"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(83)96010-X"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0076-6879(83)96057-3"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.108.3.789"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.113.1.25"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1982.tb06650.x"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2430277"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.102.6.2169"
    },
    {
      "key": "e_1_2_1_30_1",
      "first-page": "155",
      "volume": "55",
      "author": "Gething M.-J.",
      "year": "1989",
      "journal-title": "Biochem. Soc. Symp."
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj2540743"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1994.tb06788.x"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(94)00367-X"
    }
  ],
  "container-title": "FEBS Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2895%2900223-V",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(95)00223-V",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2895%2900223-V",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        16
      ]
    ],
    "date-time": "2023-09-16T05:55:28Z",
    "timestamp": 1694843728000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1016/0014-5793%2895%2900223-V"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        4,
        3
      ]
    ]
  },
  "references-count": 32,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1995,
          4,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1016/0014-5793(95)00223-V"
  ],
  "URL": "http://dx.doi.org/10.1016/0014-5793(95)00223-V",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        1995,
        4,
        3
      ]
    ]
  }
}