DOI: 10.1016/0014-5793(94)01060-9. A new additive for protein crystallization

A new additive for protein crystallization

10.1016/0014-5793(94)01060-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The potential usefulness of the new zwitterionic solubilizing agent, dimethyl ethylammonium propane sulfonate (NDSB195), in protein crystallization was shown using hen egg‐white lysozyme. In the presence of this agent, highly diffracting crystals were obtained using ammonium sulphate as a precipitant, whereas in its absence only amorphous precipitates were obtained. The crystals possess a triclinic unit cell not previously described and diffract to a resolution of 2 Å. To ascertain that the new reagent had not produced significant changes in the protein fold the structure was determined to a resolution of 2.6 Å. Only minor differences were observed (notably in regions of crystal contacts) with the known tetragonal lysozyme structure (Brookhaven Protein Data Bank entry 1HEL).

Bibliography

Vuillard, L., Rabilloud, T., Leberman, R., Berthet-Colominas, C., & Cusack, S. (1994). A new additive for protein crystallization. FEBS Letters, 353(3), 294â296. Portico.

Authors 5

Laurent Vuillard (first)
Thierry Rabilloud (additional)
Reuben Leberman (additional)
Carmen Berthet-Colominas (additional)
Stephen Cusack (additional)

References 13 Referenced 24

10.1016/S0022-2836(05)80130-5
10.1016/S0006-3495(90)82513-3
10.1016/0022-2836(92)91035-N
10.1016/S0021-9258(19)85005-6
10.1107/S0021889888007903
10.1107/S0108768189009195 / Acta Cryst. by Ramanadham M. (1990)
10.1107/S0108768189009195
10.1107/S0108767387098787
10.1126/science.235.4787.458
10.1107/S0021889878013308
10.1107/S0108767393012140 / Acta Cryst. by Ries-Kautt M. (1994)
10.1016/S0021-9258(19)50095-3
{'key': 'e_1_2_1_14_1', 'first-page': '250', 'volume': '50', 'author': 'Harata K.', 'year': '1994', 'journal-title': 'Acta Cryst.'} / Acta Cryst. by Harata K. (1994)

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 8:44 a.m.)
Indexed	1 year, 6 months ago (Feb. 10, 2024, 1:42 p.m.)
Issued	30 years, 10 months ago (Oct. 24, 1994)
Published	30 years, 10 months ago (Oct. 24, 1994)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	30 years, 10 months ago (Oct. 24, 1994)

Funders 0

None

BibTeX

@article{Vuillard_1994, title={A new additive for protein crystallization}, volume={353}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(94)01060-9}, DOI={10.1016/0014-5793(94)01060-9}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Vuillard, Laurent and Rabilloud, Thierry and Leberman, Reuben and Berthet-Colominas, Carmen and Cusack, Stephen}, year={1994}, month=oct, pages={294–296} }

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