DOI: 10.1016/0014-5793(94)01008-0. Molecular cloning and chromosomal localization of the human thrombopoietin gene

Molecular cloning and chromosomal localization of the human thrombopoietin gene

10.1016/0014-5793(94)01008-0

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The complete gene for human thrombopoietin (TPO) has been cloned by screening a human genomic library using human TPO cDNA as a probe. This gene is 6.2 kb in length and contains six exons and five introns. It is shown that the human genome contains a single copy of the human TPO gene according to Southern blotting analysis. The transcription initiation site was determined by S1 nuclease mapping. The human TPO gene expressed TPO activity when transfected into COS‐1 cells. The human TPO gene has been mapped to chromosome 3q27 by in situ hybridization using a biotin‐labeled probe.

Bibliography

Sohma, Y., Akahori, H., Seki, N., Hori, T., Ogami, K., Kato, T., Shimada, Y., Kawamura, K., & Miyazaki, H. (1994). Molecular cloning and chromosomal localization of the human thrombopoietin gene. FEBS Letters, 353(1), 57â61. Portico.

Authors 9

Yoshiaki Sohma (first)
Hiromichi Akahori (additional)
Naohiko Seki (additional)
Tada-aki Hori (additional)
Kinya Ogami (additional)
Takashi Kato (additional)
Yoshihiro Shimada (additional)
Kazuo Kawamura (additional)
Hiroshi Miyazaki (additional)

References 20 Referenced 179

{'key': 'e_1_2_1_2_1', 'first-page': '141', 'volume': '15', 'author': 'Levin J.', 'year': '1989', 'journal-title': 'Blood Cells'} / Blood Cells by Levin J. (1989)
Kato T. Ogami K. Shimada Y. Iwamatsu A. Sohma Y. Akahori H. Horie K. Kokubo A. Kudo Y. Maeda E. Kobayashi K. Ohashi H. Ozawa T. Inoue H. Kawamura K. and Miyazaki H. (in preparation).
10.1038/369533a0
10.1016/0092-8674(94)90450-2
10.1182/blood.V61.5.1027.1027
10.1016/0165-4608(82)90080-2
10.1182/blood.V68.3.652.652
1989 Cold Spring Harbor Lab. Press Plainview NY J. Sambrook E.F. Fritsh T. Maniatis Molecular Cloning: A Laboratory Manual
10.1016/S0021-9258(17)42151-X
10.1073/pnas.91.1.158
10.1093/nar/11.5.1295
1987 John Wiley and Sons New York F.M. Ausubel R. Brent R.E. Kingston D.D. Moore J.A. Smith J.G. Seidman K. Struhl Current Protocols in Molecular Biology
10.1007/BF00205165
10.1007/BF00204942
10.1007/BF00274143
10.1007/BF00193577
10.1159/000133423
10.1073/pnas.75.10.4853
10.1111/j.1365-2141.1985.tb02883.x
10.1111/j.1365-2141.1989.tb04269.x

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 1:23 p.m.)
Indexed	1 week, 5 days ago (Aug. 23, 2025, 12:58 a.m.)
Issued	30 years, 10 months ago (Oct. 10, 1994)
Published	30 years, 10 months ago (Oct. 10, 1994)
Published Online	23 years, 10 months ago (Oct. 18, 2001)
Published Print	30 years, 10 months ago (Oct. 10, 1994)

Funders 0

None

BibTeX

@article{Sohma_1994, title={Molecular cloning and chromosomal localization of the human thrombopoietin gene}, volume={353}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(94)01008-0}, DOI={10.1016/0014-5793(94)01008-0}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Sohma, Yoshiaki and Akahori, Hiromichi and Seki, Naohiko and Hori, Tada-aki and Ogami, Kinya and Kato, Takashi and Shimada, Yoshihiro and Kawamura, Kazuo and Miyazaki, Hiroshi}, year={1994}, month=oct, pages={57–61} }

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