DOI: 10.1016/0014-5793(94)00982-1. Purification and spectroscopic characterization of a recombinant amino‐terminal polypeptide fragment of mouse epithelial cadherin

Purification and spectroscopic characterization of a recombinant amino‐terminal polypeptide fragment of mouse epithelial cadherin

10.1016/0014-5793(94)00982-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Cadherins are a family of Ca2+‐dependent cell adhesion molecules containing four extracellular tandem repeats each of 110 amino acids. The most amino‐terminal repeat is believed to confer the specificity of cell adhesion. A polypeptide containing the amino‐terminal repeat of mouse epithelial cadherin has been over‐expressed in E. coli and purified to homogeneity. This polypeptide binds Ca2+ with a dissociation constant of 1.6 × 10−4M. CD and NMR experiments indicate that the polypeptide adopts a predominantly β‐sheet conformation and that binding of Ca2+ induces only small conformational changes.

Bibliography

I. Tong, K., Yau, P., Overduin, M., Bagby, S., Porumb, T., Takeichi, M., & Ikura, M. (1994). Purification and spectroscopic characterization of a recombinant aminoâterminal polypeptide fragment of mouse epithelial cadherin. FEBS Letters, 352(3), 318â322. Portico.

Authors 7

Kit I. Tong (first)
Patrick Yau (additional)
Michael Overduin (additional)
Stefan Bagby (additional)
Tudor Porumb (additional)
Masatoshi Takeichi (additional)
Mitsuhiko Ikura (additional)

References 34 Referenced 22

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 5:12 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:44 a.m.)
Indexed	1 year, 10 months ago (Oct. 31, 2023, 6:11 p.m.)
Issued	30 years, 11 months ago (Oct. 3, 1994)
Published	30 years, 11 months ago (Oct. 3, 1994)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	30 years, 11 months ago (Oct. 3, 1994)

Funders 0

None

BibTeX

@article{I_Tong_1994, title={Purification and spectroscopic characterization of a recombinant amino‐terminal polypeptide fragment of mouse epithelial cadherin}, volume={352}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(94)00982-1}, DOI={10.1016/0014-5793(94)00982-1}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={I. Tong, Kit and Yau, Patrick and Overduin, Michael and Bagby, Stefan and Porumb, Tudor and Takeichi, Masatoshi and Ikura, Mitsuhiko}, year={1994}, month=oct, pages={318–322} }

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