DOI: 10.1016/0014-5793(94)00908-2. Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells

Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells

10.1016/0014-5793(94)00908-2

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Using the baculovirus expression system the gene products of human tap1 and tap2 were over‐expressed as wild‐type as well as oligohistidine fusion proteins in Spodoptera frugiperda (Sf9) insect cells. Both gene products were co‐expressed within the same cells and were found enriched in microsomal membranes. Immunoprecipitation and immobilized metal affinity chromatography revealed complex formation between TAP1 and TAP2. The expressed TAP complex was shown to be functional by peptide translocation into microsomes of Sf9 cells. Peptide transport strictly requires TAP1 and TAP2 as well as ATP. For the first time the functional expression of the human TAP complex in insect cells has been demonstrated, indicating that additional cofactors of a highly developed immune system are not essential for peptide transport across microsomal membranes.

Bibliography

Meyer, T. H., van Endert, P. M., Uebel, S., Ehring, B., & TampÃ©, R. (1994). Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells. FEBS Letters, 351(3), 443â447. Portico.

Authors 5

Thomas H. Meyer (first)
Peter M. van Endert (additional)
Stephan Uebel (additional)
Bettina Ehring (additional)
Robert Tampé (additional)

References 43 Referenced 169

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{'key': 'e_1_2_1_40_1', 'author': 'vanEndert P.M.', 'year': '1994', 'journal-title': 'Immunity'} / Immunity by vanEndert P.M. (1994)
10.1073/pnas.74.12.5463
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{'key': 'e_1_2_1_43_1', 'author': "O'Reilly D.R.", 'year': '1992', 'journal-title': 'Baculovirus Expression Vectors'} / Baculovirus Expression Vectors by O'Reilly D.R. (1992)
1988 Cold Spring Harbour Laboratory E. Harlow D. Lane Antibodies

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:40 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 4:05 a.m.)
Indexed	3 weeks, 3 days ago (Aug. 5, 2025, 8:20 a.m.)
Issued	30 years, 11 months ago (Sept. 12, 1994)
Published	30 years, 11 months ago (Sept. 12, 1994)
Published Online	23 years, 10 months ago (Oct. 18, 2001)
Published Print	30 years, 11 months ago (Sept. 12, 1994)

Funders 0

None

BibTeX

@article{Meyer_1994, title={Functional expression and purification of the ABC transporter complex associated with antigen processing (TAP) in insect cells}, volume={351}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(94)00908-2}, DOI={10.1016/0014-5793(94)00908-2}, number={3}, journal={FEBS Letters}, publisher={Wiley}, author={Meyer, Thomas H. and van Endert, Peter M. and Uebel, Stephan and Ehring, Bettina and Tampé, Robert}, year={1994}, month=sep, pages={443–447} }

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