Crossref journal-article
Wiley
FEBS Letters (311)
Abstract

A cross‐linked product between the D1 and D2 subunits was generated by treating isolated spinach PS II reaction center with a bifunctional cross‐linker, 1,6‐hexamethylene diisocyanate. To obtain information on the contact site(s) between D1 and D2 proteins, the cross‐linked product was cleaved by CNBr, the resultant fragments were separated using a reverse‐phase HPLC, and then the partial sequence of the cross‐linked polypeptide fragments were determined. By comparing the results with the deduced amino acid sequence of the D1 and D2 proteins from spinach, it is concluded that the C‐terminal domains of the D1 subunit (D308‐A334) and that of the D2 subunit (Y297‐L353) are in close proximity.

Bibliography

Tomo, T., & Satoh, K. (1994). Nearest neighbor analysis of D1 and D2 subunits in the photosystem II reaction center using a bifunctional cross‐linker, hexamethylene diisocyanate. FEBS Letters, 351(1), 27–30. Portico.

Authors 2
  1. Tatsuya Tomo (first)
  2. Kimiyuki Satoh (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 25, 2002, 3:40 a.m.)
Deposited 1 year, 11 months ago (Sept. 16, 2023, 10:08 p.m.)
Indexed 1 year, 4 months ago (April 7, 2024, 9:16 a.m.)
Issued 31 years ago (Aug. 29, 1994)
Published 31 years ago (Aug. 29, 1994)
Published Online 23 years, 10 months ago (Oct. 19, 2001)
Published Print 31 years ago (Aug. 29, 1994)
Funders 0

None

@article{Tomo_1994, title={Nearest neighbor analysis of D1 and D2 subunits in the photosystem II reaction center using a bifunctional cross‐linker, hexamethylene diisocyanate}, volume={351}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(94)00803-5}, DOI={10.1016/0014-5793(94)00803-5}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Tomo, Tatsuya and Satoh, Kimiyuki}, year={1994}, month=aug, pages={27–30} }