DOI: 10.1016/0014-5793(94)00664-4. The occupancy of two distinct conformations by active‐site histidine‐119 in crystals of ribonuclease is modulated by pH

The occupancy of two distinct conformations by active‐site histidine‐119 in crystals of ribonuclease is modulated by pH

10.1016/0014-5793(94)00664-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Structures of a semisynthetic RNase have been obtained to a resolution of 2.0 Å at pH values of 5.2, 6.5, 7.5, and 8.8, respectively. The principle structural transformation occurring over this pH range is the conversion of the side chain of active site residue His‐119 from one conformation (X 1 = −43° to −57°) at low pH to another (X 1 = + 159° to + 168°) at higher pH values. On the basis of this observation, a model is proposed that reconciles the disparate pK values for His‐119 in the enzyme‐substrate complex that have been deduced from kinetic studies and from proton NMR measurements in the presence of pseudosubstrates.

Bibliography

J. de Mel, V. S., Doscher, M. S., Martin, P. D., & Edwards, B. F. P. (1994). The occupancy of two distinct conformations by activeâsite histidineâ119 in crystals of ribonuclease is modulated by pH. FEBS Letters, 349(1), 155â160. Portico.

Authors 4

V.Srini J. de Mel (first)
Marilynn S. Doscher (additional)
Philip D. Martin (additional)
Brian F.P. Edwards (additional)

References 49 Referenced 19

10.1107/S0567740882008346
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1971 Academic Press New York F.M. Richards H.W. Wyckoff P.D. Boyer The Enzymes 647 806
1982 Academic Press New York P. Blackburn S. Moore P.D. Boyer The Enzymes 317 433
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10.1111/j.1432-1033.1978.tb12350.x

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 5:35 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 8:07 p.m.)
Indexed	1 year, 10 months ago (Oct. 16, 2023, 9:13 a.m.)
Issued	31 years, 1 month ago (July 25, 1994)
Published	31 years, 1 month ago (July 25, 1994)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	31 years, 1 month ago (July 25, 1994)

Funders 0

None

BibTeX

@article{J_de_Mel_1994, title={The occupancy of two distinct conformations by active‐site histidine‐119 in crystals of ribonuclease is modulated by pH}, volume={349}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(94)00664-4}, DOI={10.1016/0014-5793(94)00664-4}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={J. de Mel, V.Srini and Doscher, Marilynn S. and Martin, Philip D. and Edwards, Brian F.P.}, year={1994}, month=jul, pages={155–160} }

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