DOI: 10.1016/0014-5793(93)80097-e. Divergent effects of fluoroaluminates on the peptide chain elongation factors EF‐Tu and EF‐G as members of the GTPase superfamily

Divergent effects of fluoroaluminates on the peptide chain elongation factors EF‐Tu and EF‐G as members of the GTPase superfamily

10.1016/0014-5793(93)80097-e

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Fluoroaluminates are thought to mimic the γ‐phosphate of GTP and thus, together with GDP, perturb the functioning of heterotrimeric GTP‐binding G‐proteins. Here we show they do inhibit the ribosome‐stimulated GTPase activity of EF‐G from Escherichia coli via the formation of a stable complex with EF‐G·GDP and ribosomes. In contrast, no perturbed interactions were observed in a similar ribosomal complex with EF‐Tu. Interestingly, in the absence of ribosomes both EF‐Tu and EF‐G remain totally unaffected by fluoroaluminates. For members of the GTPase superfamily such differential effects have not been described before.

Bibliography

Mesters, J. R., de Graaf, J. M., & Kraal, B. (1993). Divergent effects of fluoroaluminates on the peptide chain elongation factors EFâTu and EFâG as members of the GTPase superfamily. FEBS Letters, 321(2â3), 149â152. Portico.

Authors 3

Jeroen R. Mesters (first)
J.Martien de Graaf (additional)
Barend Kraal (additional)

References 25 Referenced 17

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 5:09 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 3:17 p.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 12:28 a.m.)
Issued	32 years, 4 months ago (April 26, 1993)
Published	32 years, 4 months ago (April 26, 1993)
Published Online	23 years, 10 months ago (Nov. 1, 2001)
Published Print	32 years, 4 months ago (April 26, 1993)

Funders 0

None

BibTeX

@article{Mesters_1993, title={Divergent effects of fluoroaluminates on the peptide chain elongation factors EF‐Tu and EF‐G as members of the GTPase superfamily}, volume={321}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(93)80097-e}, DOI={10.1016/0014-5793(93)80097-e}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Mesters, Jeroen R. and de Graaf, J.Martien and Kraal, Barend}, year={1993}, month=apr, pages={149–152} }

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