Abstract
Photoreduction of NAD has been accomplished by a system consisting of the NAD‐dependent hydrogenase fromAlcaligenes eutrophusimmobilized on CdS particles with formate as artificial electron donor. Enzymatically active NADH is formed under illumination of this system by visible light. Accumulation of the coenzyme dimer (NAD)2was not detected. NAD photoreduction is supposed to proceed via the direct electron transfer from the semiconductor to the enzyme electron transport chain. However, NADH formation as a result of hydrogenase interaction with anion‐radicals (CO.−2)‐formed in the course of formate photooxidation cannot at present be excluded.
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Dates
Type | When |
---|---|
Created | 23 years, 1 month ago (July 25, 2002, 3:42 a.m.) |
Deposited | 8 months, 3 weeks ago (Dec. 2, 2024, 9:57 p.m.) |
Indexed | 8 months, 3 weeks ago (Dec. 3, 2024, 12:21 a.m.) |
Issued | 33 years, 1 month ago (July 20, 1992) |
Published | 33 years, 1 month ago (July 20, 1992) |
Published Online | 23 years, 8 months ago (Dec. 17, 2001) |
Published Print | 33 years, 1 month ago (July 20, 1992) |
@article{Shumilin_1992, title={Photogeneration of NADH under coupled action of CdS semiconductor and hydrogenase fromAlcaligenes eutrophuswithout exogenous mediators}, volume={306}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(92)80982-m}, DOI={10.1016/0014-5793(92)80982-m}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Shumilin, I.A. and Nikandrov, V.V. and Popov, V.O. and Krasnovsky, A.A.}, year={1992}, month=jul, pages={125–128} }