DOI: 10.1016/0014-5793(92)80730-5. Membrane inserted APP fragments containing the βA4 sequence of Alzheimer's disease do not aggregate

Membrane inserted APP fragments containing the βA4 sequence of Alzheimer's disease do not aggregate

10.1016/0014-5793(92)80730-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Previously we have shown that the COOH‐terminal fragment (A4CT) of the Alzheimer amyloid protein precursor (APP), which at the NH2‐terminus carries the sequence of the amyloid βA4 protein, forms highly insoluble aggregates [EMBO J. (1988) 7, 949–957]. Here we report that aggregation is prevented if A4CT is expressed in vitro with a signal sequence at the NH2‐terminus (SPA4CT) under conditions which allow membrane insertion. Aggregates from SPA4CT are obtained after removal of membranes by chloroform/methanol extraction or heating.

Bibliography

Dyrks, T., Dyrks, E., Masters, C., & Beyreuther, K. (1992). Membrane inserted APP fragments containing the Î²A4 sequence of Alzheimerâs disease do not aggregate. FEBS Letters, 309(1), 20â24. Portico.

Authors 4

Thomas Dyrks (first)
Elke Dyrks (additional)
Colin Masters (additional)
Konrad Beyreuther (additional)

References 24 Referenced 29

10.1002/j.1460-2075.1988.tb02900.x
10.1038/209109a0
10.1016/0006-291X(84)91209-9
10.1016/S0006-291X(84)80190-4
10.1073/pnas.82.12.4245
10.1002/j.1460-2075.1985.tb04000.x
10.1111/j.1750-3639.1991.tb00667.x
10.1038/325733a0
10.1038/331528a0
10.1038/331530a0
10.1038/331525a0
10.1016/0092-8674(89)90177-3
10.1126/science.1691865
10.1126/science.2111583
10.1126/science.1738846
1989 Cold Spring Harbor Laboratory Press NY T. Maniatis E.F. Fritch J. Sambrook Molecular Cloning: A Laboratory Manual
10.1016/0092-8674(84)90268-X
10.1016/S0092-8674(85)80025-8
10.1083/jcb.93.1.97
1982 Elsevier Amsterdam M. Kates Techniques of Lipidology: Laboratory Techniques in Biochemistry and Molecular Biology
{'key': 'e_1_2_1_22_1', 'author': 'Dyrks T.', 'year': '1992', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. by Dyrks T. (1992)
10.1038/227680a0
10.1083/jcb.93.1.97
{'key': 'e_1_2_1_25_1', 'first-page': '741', 'volume': '14', 'author': 'Arai H.', 'year': '1987', 'journal-title': 'Biochem. Int.'} / Biochem. Int. by Arai H. (1987)

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:42 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 7 a.m.)
Indexed	1 year, 9 months ago (Nov. 22, 2023, 1:02 p.m.)
Issued	32 years, 11 months ago (Aug. 31, 1992)
Published	32 years, 11 months ago (Aug. 31, 1992)
Published Online	23 years, 7 months ago (Jan. 16, 2002)
Published Print	32 years, 11 months ago (Aug. 31, 1992)

Funders 0

None

BibTeX

@article{Dyrks_1992, title={Membrane inserted APP fragments containing the βA4 sequence of Alzheimer’s disease do not aggregate}, volume={309}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(92)80730-5}, DOI={10.1016/0014-5793(92)80730-5}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Dyrks, Thomas and Dyrks, Elke and Masters, Colin and Beyreuther, Konrad}, year={1992}, month=aug, pages={20–24} }

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