DOI: 10.1016/0014-5793(92)80619-r. The identification and characterisation of an actin‐binding site in α‐actinin by mutagenesis

The identification and characterisation of an actin‐binding site in α‐actinin by mutagenesis

10.1016/0014-5793(92)80619-r

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

We have shown previously that the N‐terminal actin‐binding domain of α‐actinin retains activity when expressed in E. coli as a fusion protein with glutathione‐S‐transferase. In the present study we have made a series of N‐ and C‐terminal deletions within this domain and show that an actin‐binding site is contained within residues 120–134. Amino acid substitutions within this region indicate that several highly conserved hydrophobic residues are involved in binding to F‐actin. The hypothesis that the interaction between α‐actinin and F‐actin is predominantly hydrophobic in nature is supported by the observation that binding is relatively independent of salt concentration.

Bibliography

Kuhlman, P. A., Hemmings, L., & Critchley, D. R. (1992). The identification and characterisation of an actinâbinding site in Î±âactinin by mutagenesis. FEBS Letters, 304(2â3), 201â206. Portico.

Authors 3

Philip A. Kuhlman (first)
Lance Hemmings (additional)
David R. Critchley (additional)

References 19 Referenced 58

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:42 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:07 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:26 a.m.)
Issued	33 years, 2 months ago (June 15, 1992)
Published	33 years, 2 months ago (June 15, 1992)
Published Online	23 years, 7 months ago (Jan. 16, 2002)
Published Print	33 years, 2 months ago (June 15, 1992)

Funders 0

None

BibTeX

@article{Kuhlman_1992, title={The identification and characterisation of an actin‐binding site in α‐actinin by mutagenesis}, volume={304}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(92)80619-r}, DOI={10.1016/0014-5793(92)80619-r}, number={2–3}, journal={FEBS Letters}, publisher={Wiley}, author={Kuhlman, Philip A. and Hemmings, Lance and Critchley, David R.}, year={1992}, month=jun, pages={201–206} }

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