DOI: 10.1016/0014-5793(92)80086-v. Evidence for non‐cysteinyl coordination of the [2Fe‐2S] cluster in <i>Escherichia coli</i> succinate dehydrogenase

Evidence for non‐cysteinyl coordination of the [2Fe‐2S] cluster in Escherichia coli succinate dehydrogenase

10.1016/0014-5793(92)80086-v

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The consequences of replacing Cys65 in the FrdB subunit of Escherichia coli fumarate reductase by Asp or Ala have been investigated in terms of bacterial growth, enzymatic activity, and the EPR/redox properties of the [2Fe‐2S] cluster. An aspartic acid residue occupies the equivalent position in E. coli succinate dehydrogenase, and the FrdBCys65Asp mutation has little effect on cell growth, enzyme activity or the physical properties of the Frd [2Fe‐2S] cluster. In contrast, the [2Fe‐2S] cluster was not observed in the FrdBCys65Ala mutant showing that a coordinating residue is required at this position for assembly of this cluster and significant levels or enzymatic activity. These results support the presence of one non‐cysteinyl, oxygenic ligand for the [2Fe‐2S] cluster in E. coli succinate dehydrogenase.

Bibliography

Werth, M. T., Sices, H., Cecchini, G., SchrÃ¶der, I., Lasage, S., Gunsalus, R. P., & Johnson, M. K. (1992). Evidence for nonâcysteinyl coordination of the [2Feâ2S] cluster in Escherichia coli succinate dehydrogenase. FEBS Letters, 299(1), 1â4. Portico.

Authors 7

Mark T. Werth (first)
Harry Sices (additional)
Gary Cecchini (additional)
Imke Schröder (additional)
Susanne Lasage (additional)
Robert P. Gunsalus (additional)
Michael K. Johnson (additional)

References 14 Referenced 27

10.1016/B978-0-12-152515-6.50006-0
1991 CRC Presss Boca Raton B.A.C. Ackrell M.K. Johnson R.P. Gunsalus G. Cecchini F. Muller Chemistry and Biochemistry of Flavoenzymes 229 297
10.1073/pnas.87.22.8965
10.1042/bj2230507
10.1016/S0021-9258(17)39384-5
10.1016/S0021-9258(19)81666-6
10.1016/S0021-9258(18)92737-7
10.1016/S0021-9258(17)36012-X
10.1073/pnas.83.23.8898
10.1016/S0021-9258(17)38772-0
10.1016/0006-291X(85)91303-8
10.1016/0167-4838(86)90264-5
10.1042/bj2640275
10.1016/S0021-9258(19)38921-5

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:42 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:04 a.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 1:23 p.m.)
Issued	33 years, 5 months ago (March 2, 1992)
Published	33 years, 5 months ago (March 2, 1992)
Published Online	23 years, 7 months ago (Jan. 16, 2002)
Published Print	33 years, 5 months ago (March 2, 1992)

Funders 0

None

BibTeX

@article{Werth_1992, title={Evidence for non‐cysteinyl coordination of the [2Fe‐2S] cluster in Escherichia coli succinate dehydrogenase}, volume={299}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(92)80086-v}, DOI={10.1016/0014-5793(92)80086-v}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Werth, Mark T. and Sices, Harry and Cecchini, Gary and Schröder, Imke and Lasage, Susanne and Gunsalus, Robert P. and Johnson, Michael K.}, year={1992}, month=mar, pages={1–4} }

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