DOI: 10.1016/0014-5793(92)80025-c. Characterization and differential expression of protein kinase C isoforms in PC12 cells Differentiation parallels an increase in PKC beta<sub>11</sub>

Characterization and differential expression of protein kinase C isoforms in PC12 cells Differentiation parallels an increase in PKC beta11

10.1016/0014-5793(92)80025-c

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Nerve growth factor (NGF) treatment of PC12 cells induced a 2.8‐fold increase in protein kinase C activity concomitant with differentiation and acquisition or neurites. PKC protein isoforms were separated by sequential chromatography on DEAE‐Sephacel/hydroxylapatite. A broad peak or PKC activity eluted which corresponded to the alpha PKC isoform. In control cells, message for all six PKC isoforms was detected and expressed as epsilon>zeta=gamma>delta>beta>alpha. Western blot or whole cell lysates revealed a large increase in the beta11, while slight changes were observed for the other five PKC isoforms during treatment (12‐14 days) with NGF (50 ng/ml). In parallel, coordinate changes in the expression of the individual transcripts for the six isoforms occurred during NGF treatment. Induction and accumulation of PKC beta11 may play a role in maintenance or neuronal morphology.

Bibliography

Wooten, M. W., Seibenhener, M. L., Soh, Y., Ewald, S. J., White, K. R., Lloyd, E. D., Olivier, A., & Parker, P. J. (1992). Characterization and differential expression of protein kinase C isoforms in PC12 cells Differentiation parallels an increase in PKC beta11. FEBS Letters, 298(1), 74â78. Portico.

Authors 8

Marie W. Wooten (first)
M.Lamar Seibenhener (additional)
Yunjo Soh (additional)
Sandra J. Ewald (additional)
Kimberly R. White (additional)
Elizabeth D. Lloyd (additional)
Andree Olivier (additional)
Peter J. Parker (additional)

References 28 Referenced 46

10.1038/334661a0
10.1016/0014-5793(90)81284-U
10.1016/S0006-291X(88)80461-3
{'key': 'e_1_2_1_5_1', 'first-page': '8538', 'volume': '83', 'author': 'Huang K.-P.', 'year': '1986', 'journal-title': 'Proc. Natl. Acad. Sci. USA'} / Proc. Natl. Acad. Sci. USA by Huang K.-P. (1986)
10.1126/science.3576226
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10.1016/S0021-9258(18)69085-4
10.1016/S0021-9258(18)51481-2
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10.1523/JNEUROSCI.08-02-00715.1988
10.1016/0006-8993(91)91337-Z
10.1016/0014-4827(91)90096-D
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{'key': 'e_1_2_1_20_1', 'author': 'Ways K.', 'year': '1991', 'journal-title': 'J. Biol, Chem.'} / J. Biol, Chem. by Ways K. (1991)
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10.1073/pnas.88.12.5149

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:42 a.m.)
Deposited	1 year, 11 months ago (Sept. 17, 2023, 2:08 a.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:38 p.m.)
Issued	33 years, 6 months ago (Feb. 17, 1992)
Published	33 years, 6 months ago (Feb. 17, 1992)
Published Online	23 years, 7 months ago (Jan. 16, 2002)
Published Print	33 years, 6 months ago (Feb. 17, 1992)

Funders 0

None

BibTeX

@article{Wooten_1992, title={Characterization and differential expression of protein kinase C isoforms in PC12 cells Differentiation parallels an increase in PKC beta11}, volume={298}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(92)80025-c}, DOI={10.1016/0014-5793(92)80025-c}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Wooten, Marie W. and Seibenhener, M.Lamar and Soh, Yunjo and Ewald, Sandra J. and White, Kimberly R. and Lloyd, Elizabeth D. and Olivier, Andree and Parker, Peter J.}, year={1992}, month=feb, pages={74–78} }

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