DOI: 10.1016/0014-5793(91)81359-g. Phosphorylation of α‐crystallin B in Alexander's disease brain

Phosphorylation of α‐crystallin B in Alexander's disease brain

10.1016/0014-5793(91)81359-g

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The phosphorylation of α‐crystallin B was studied in homogenates of autopsy samples of brain tissue from patients with Alexander's disease, a condition characterized by over‐expression of this protein. After incubation in the presence of [γ‐32P]ATP and cAMP the homogenates were analyzed by two‐dimensional electrophoresis, (isoelectric focusing followed by SDS‐PAGE). Three major polypeptides having the same molecular weight as bovine lens α‐crystallin B and pIs 7.1, 6.9 and 6.7 were detected in the Coomassie blue stained gels. These three polypeptides were recognized by an α‐crystallin B‐specific antiserum in Western blots. The polypeptides with pIs 7.1 and 6.7 co‐migrated in isoelectric focusing gels with bovine lens αB and its phosphorylated form αBp, respectively. Radioautography of the two‐dimensional gels demonstrated the presence of 32P in the most acidic polypeptide. The results demonstrate the occurrence of αB phosphorylation in Alexander's disease brain tissue.

Bibliography

Mann, E., McDermott, M. J., Goldman, J., Chiesa, R., & Spector, A. (1991). Phosphorylation of Î±âcrystallin B in Alexanderâs disease brain. FEBS Letters, 294(1â2), 133â136. Portico.

Authors 5

Eric Mann (first)
Martin J. McDermott (additional)
James Goldman (additional)
Raúl Chiesa (additional)
Abraham Spector (additional)

References 35 Referenced 28

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Dates

Type	When
Created	23 years ago (July 25, 2002, 4:06 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:58 a.m.)
Indexed	2 months, 1 week ago (June 12, 2025, 5:43 a.m.)
Issued	33 years, 8 months ago (Dec. 2, 1991)
Published	33 years, 8 months ago (Dec. 2, 1991)
Published Online	23 years, 4 months ago (April 12, 2002)
Published Print	33 years, 8 months ago (Dec. 2, 1991)

Funders 0

None

BibTeX

@article{Mann_1991, title={Phosphorylation of α‐crystallin B in Alexander’s disease brain}, volume={294}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(91)81359-g}, DOI={10.1016/0014-5793(91)81359-g}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Mann, Eric and McDermott, Martin J. and Goldman, James and Chiesa, Raúl and Spector, Abraham}, year={1991}, month=dec, pages={133–136} }

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