DOI: 10.1016/0014-5793(91)81352-9. Identification of the phosphorylation sites of H2B histone by a catalytic fragment of p72<sup><i>syk</i></sup> from porcine spleen

Identification of the phosphorylation sites of H2B histone by a catalytic fragment of p72syk from porcine spleen

10.1016/0014-5793(91)81352-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Phosphorylated sites of calf thymus H2B histone were investigated with a catalytic fragment of 72 kDa protein‐tyrosine kinase (p72 syk ). Three of five tyrosine residues in H2B histone can be phosphorylated by this kinase. In this analysis, H2B histone was thoroughly phosphorylated in vitro with [γ‐32P]ATP and the kinase, and then digested with a lysylendopeptidase. The resulting radioactive phosphopeptides were separated by a reverse‐phase column on high performance liquid chromatography. Subsequent sequential Edman degradation of the purified phosphopeptides revealed that 40Y, 83Y and 121Y were phosphorylated. 121Y is the major phosphorylated residue in H2B histone. No phosphorylation was detected in 37Y and 42Y. Although the consensus sequence was not defined from these analyses, our data suggest that higher‐order structure(s) in addition to primary one may participate in recognition of H2B histone by this protein kinase.

Bibliography

Sakai, K., Tanaka, Y., Asahi, M., Shimomura, R., Taniguchi, T., Hashimoto, E., & Yamamura, H. (1991). Identification of the phosphorylation sites of H2B histone by a catalytic fragment of p72syk from porcine spleen. FEBS Letters, 294(1â2), 104â108. Portico.

Authors 7

Keiko Sakai (first)
Yukie Tanaka (additional)
Momoyo Asahi (additional)
Reiko Shimomura (additional)
Takanobu Taniguchi (additional)
Eikichi Hashimoto (additional)
Hirohei Yamamura (additional)

References 21 Referenced 5

10.1126/science.3291115
10.1016/0092-8674(91)90639-G
10.1016/S0006-291X(88)80401-7
10.1111/j.1432-1033.1990.tb15433.x
10.1016/S0021-9258(18)98478-4
10.1016/0006-291X(89)92827-1
10.1073/pnas.79.4.973
10.1111/j.1432-1033.1990.tb19468.x
1990 CRC Press Boca Raton FL R.L. Geahlen M.L. Harrison B.E. Kemp Peptide and Protein Phosphorylation 239 253
10.1016/S0021-9258(18)32267-1
1984 B.M. Sefton T. Hunter P.A. Greengard G.A. Robison Advances Cyclic Nucleotide and Protein Phosphorylation Research 195 226
10.1073/pnas.79.5.1443
10.1016/S0021-9258(18)60671-4
10.1016/S0021-9258(20)81856-0
10.1016/S0021-9258(18)61100-7
10.1016/S0021-9258(18)67542-8
10.1016/0020-711X(90)90041-Z
10.1016/S0021-9258(17)41009-X
{'key': 'e_1_2_1_20_1', 'first-page': '45', 'volume': '47', 'author': 'Chou P.Y.', 'year': '1978', 'journal-title': 'Adv. Enzymol.'} / Adv. Enzymol. by Chou P.Y. (1978)
10.1146/annurev.bi.56.070187.003031
10.1016/0014-5793(86)81388-6

Dates

Type	When
Created	23 years ago (July 25, 2002, 4:06 p.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:59 a.m.)
Indexed	1 year, 11 months ago (Sept. 17, 2023, 1:10 a.m.)
Issued	33 years, 8 months ago (Dec. 2, 1991)
Published	33 years, 8 months ago (Dec. 2, 1991)
Published Online	23 years, 4 months ago (April 12, 2002)
Published Print	33 years, 8 months ago (Dec. 2, 1991)

Funders 0

None

BibTeX

@article{Sakai_1991, title={Identification of the phosphorylation sites of H2B histone by a catalytic fragment of p72syk from porcine spleen}, volume={294}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(91)81352-9}, DOI={10.1016/0014-5793(91)81352-9}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Sakai, Keiko and Tanaka, Yukie and Asahi, Momoyo and Shimomura, Reiko and Taniguchi, Takanobu and Hashimoto, Eikichi and Yamamura, Hirohei}, year={1991}, month=dec, pages={104–108} }

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