DOI: 10.1016/0014-5793(90)81505-i. Subcellular distribution of α, and β subunit proteins of Ca<sup>2+</sup> /calmodulin‐dependent protein kinase II expressed in Chinese hamster ovary cells

Subcellular distribution of α, and β subunit proteins of Ca2+ /calmodulin‐dependent protein kinase II expressed in Chinese hamster ovary cells

10.1016/0014-5793(90)81505-i

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

When two cDNAs respectively encoding the entire coding regions of α and β subunits of Ca2+ /calmodulin‐dependent protein kinase II (CaM kinase II) were introduced into Chinese hamster ovary cells, the expressed α and β subunits were differently associated with subcellular structure. Although α subunit was loosely associated with subcellular structure, about 80% of CaM kinase II acitivity of α subunit was found in soluble fraction. More than 50% of the β subunit bound to the membrane, and the remainder was soluble but was loosely associated with subcellular structure. The relative rate of phosphorylation for substrate proteins of the β subunit bound to membrane was significantly different from that of the soluble form.

Bibliography

Yamauchi, T., Sekihara, S., & Ohsako, S. (1990). Subcellular distribution of Î±, and Î² subunit proteins of Ca2+ /calmodulinâdependent protein kinase II expressed in Chinese hamster ovary cells. FEBS Letters, 266(1â2), 55â58. Portico.

Authors 3

Takashi Yamauchi (first)
Shun-ichi Sekihara (additional)
Shunji Ohsako (additional)

References 19 Referenced 12

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 5:10 a.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 11:44 p.m.)
Indexed	1 year, 10 months ago (Oct. 23, 2023, 1:10 p.m.)
Issued	35 years, 2 months ago (June 18, 1990)
Published	35 years, 2 months ago (June 18, 1990)
Published Online	23 years, 7 months ago (Jan. 9, 2002)
Published Print	35 years, 2 months ago (June 18, 1990)

Funders 0

None

BibTeX

@article{Yamauchi_1990, title={Subcellular distribution of α, and β subunit proteins of Ca2+ /calmodulin‐dependent protein kinase II expressed in Chinese hamster ovary cells}, volume={266}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(90)81505-i}, DOI={10.1016/0014-5793(90)81505-i}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Yamauchi, Takashi and Sekihara, Shun-ichi and Ohsako, Shunji}, year={1990}, month=jun, pages={55–58} }

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