DOI: 10.1016/0014-5793(90)80704-m. ATP synthesis coupled to electron transfer from H<sub>2</sub> to the heterodisulfide of 2‐mercaptoethanesulfonate and 7‐mercaptoheptanoylthreonine phosphate in vesicle preparations of the methanogenic bacterium strain Gö1

ATP synthesis coupled to electron transfer from H2 to the heterodisulfide of 2‐mercaptoethanesulfonate and 7‐mercaptoheptanoylthreonine phosphate in vesicle preparations of the methanogenic bacterium strain Gö1

10.1016/0014-5793(90)80704-m

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Crude vesicle preparations of the methanogenic strain Gol were able to couple the reduction of the heterodisulfide of 2‐mercaptoethanesulfonate and 7‐mercaptoheptanoylthreonine phosphate (CoM‐S‐S‐HTP) by H2 with ATP formation. The rate of ATP synthesis was 1 mg protein. ATP synthesis and disulfide reduction were only observed with CoM‐S‐S‐HTP, but not with CoM‐S‐S‐CoM or HTP‐S‐S‐HTP. The methylreductase inhibitor 2‐bromoethanesulfonic acid had no effect on ATP synthesis induced by CoM‐S‐S‐HTP reduction with H2. ATP synthesis was completely inhibited by the uncoupler SF 6847 whereas the concomitant CoM‐S‐S‐HTP reduction was stimulated. The ATP synthase inhibitors DCCD and DES also inhibited ATP formation completely and decreased the CoM‐S‐S‐HTP reduction rate to 35% of the control. These inhibitory effects were abolished by addition of the uncoupler. From these results it is concluded that energy coupling between the electron transfer from H2 to the heterodisulfide and ATP synthesis occurs via a transmembrane proton gradient.

Bibliography

Peinemann, S., Hedderich, R., Blaut, M., Thauer, R. K., & Gottschalk, G. (1990). ATP synthesis coupled to electron transfer from H2 to the heterodisulfide of 2âmercaptoethanesulfonate and 7âmercaptoheptanoylthreonine phosphate in vesicle preparations of the methanogenic bacterium strain GÃ¶1. FEBS Letters, 263(1), 57â60. Portico.

Authors 5

S. Peinemann (first)
R. Hedderich (additional)
M. Blaut (additional)
R.K. Thauer (additional)
G. Gottschalk (additional)

References 14 Referenced 30

10.1111/j.1432-1033.1984.tb08178.x
10.1007/BF00403224
10.1111/j.1432-1033.1988.tb13941.x
10.1016/0006-291X(87)90389-5
10.1128/jb.170.4.1438-1444.1988
10.1111/j.1432-1033.1989.tb15192.x
10.1073/pnas.76.1.494
10.1126/science.793008
10.1515/znb-1953-1005
10.1016/0014-5793(88)81339-5
10.1111/j.1432-1033.1989.tb14990.x
10.1016/0014-5793(89)81062-2
{'key': 'e_1_2_1_14_1', 'author': 'Deppenmeier U.', 'year': '1990', 'journal-title': 'FEBS Lett.'} / FEBS Lett. by Deppenmeier U. (1990)
10.1111/j.1432-1033.1989.tb15211.x

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:44 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 7:52 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 7:05 p.m.)
Issued	35 years, 4 months ago (April 9, 1990)
Published	35 years, 4 months ago (April 9, 1990)
Published Online	23 years, 9 months ago (Nov. 12, 2001)
Published Print	35 years, 4 months ago (April 9, 1990)

Funders 0

None

BibTeX

@article{Peinemann_1990, title={ATP synthesis coupled to electron transfer from H2 to the heterodisulfide of 2‐mercaptoethanesulfonate and 7‐mercaptoheptanoylthreonine phosphate in vesicle preparations of the methanogenic bacterium strain Gö1}, volume={263}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(90)80704-m}, DOI={10.1016/0014-5793(90)80704-m}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Peinemann, S. and Hedderich, R. and Blaut, M. and Thauer, R.K. and Gottschalk, G.}, year={1990}, month=apr, pages={57–60} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        24
      ]
    ],
    "date-time": "2023-11-24T00:05:27Z",
    "timestamp": 1700784327148
  },
  "reference-count": 14,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            11,
            12
          ]
        ],
        "date-time": "2001-11-12T00:00:00Z",
        "timestamp": 1005523200000
      },
      "content-version": "vor",
      "delay-in-days": 4235,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1990,
        4,
        9
      ]
    ]
  },
  "abstract": "<jats:p>Crude vesicle preparations of the methanogenic strain Gol were able to couple the reduction of the heterodisulfide of 2\u2010mercaptoethanesulfonate and 7\u2010mercaptoheptanoylthreonine phosphate (CoM\u2010<jats:italic>S</jats:italic>\u2010<jats:italic>S</jats:italic>\u2010HTP) by H<jats:sub>2</jats:sub> with ATP formation. The rate of ATP synthesis was 1 <jats:styled-content><jats:inline-graphic xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"graphic/feb2001457939080704m-math-si1.gif\" xlink:title=\"urn:x-wiley:00145793:media:feb2001457939080704m:feb2001457939080704m-math-si1\" /></jats:styled-content> mg protein. ATP synthesis and disulfide reduction were only observed with CoM\u2010<jats:italic>S</jats:italic>\u2010<jats:italic>S</jats:italic>\u2010HTP, but not with CoM\u2010<jats:italic>S</jats:italic>\u2010<jats:italic>S</jats:italic>\u2010CoM or HTP\u2010<jats:italic>S</jats:italic>\u2010<jats:italic>S</jats:italic>\u2010HTP. The methylreductase inhibitor 2\u2010bromoethanesulfonic acid had no effect on ATP synthesis induced by CoM\u2010<jats:italic>S</jats:italic>\u2010<jats:italic>S</jats:italic>\u2010HTP reduction with H<jats:sub>2</jats:sub>. ATP synthesis was completely inhibited by the uncoupler SF 6847 whereas the concomitant CoM\u2010<jats:italic>S</jats:italic>\u2010<jats:italic>S</jats:italic>\u2010HTP reduction was stimulated. The ATP synthase inhibitors DCCD and DES also inhibited ATP formation completely and decreased the CoM\u2010<jats:italic>S</jats:italic>\u2010<jats:italic>S</jats:italic>\u2010HTP reduction rate to 35% of the control. These inhibitory effects were abolished by addition of the uncoupler. From these results it is concluded that energy coupling between the electron transfer from H<jats:sub>2</jats:sub> to the heterodisulfide and ATP synthesis occurs via a transmembrane proton gradient.</jats:p>",
  "DOI": "10.1016/0014-5793(90)80704-m",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        25
      ]
    ],
    "date-time": "2002-07-25T07:44:17Z",
    "timestamp": 1027583057000
  },
  "page": "57-60",
  "source": "Crossref",
  "is-referenced-by-count": 30,
  "title": "ATP synthesis coupled to electron transfer from H<sub>2</sub> to the heterodisulfide of 2\u2010mercaptoethanesulfonate and 7\u2010mercaptoheptanoylthreonine phosphate in vesicle preparations of the methanogenic bacterium strain G\u00f61",
  "prefix": "10.1002",
  "volume": "263",
  "author": [
    {
      "given": "S.",
      "family": "Peinemann",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "R.",
      "family": "Hedderich",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M.",
      "family": "Blaut",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "R.K.",
      "family": "Thauer",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "G.",
      "family": "Gottschalk",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2001,
        11,
        12
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1984.tb08178.x"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00403224"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1988.tb13941.x"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(87)90389-5"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.170.4.1438-1444.1988"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1989.tb15192.x"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.76.1.494"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.793008"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1515/znb-1953-1005"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(88)81339-5"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1989.tb14990.x"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(89)81062-2"
    },
    {
      "key": "e_1_2_1_14_1",
      "author": "Deppenmeier U.",
      "year": "1990",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1989.tb15211.x"
    }
  ],
  "container-title": "FEBS Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2890%2980704-M",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(90)80704-M",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2890%2980704-M",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        16
      ]
    ],
    "date-time": "2023-09-16T23:52:25Z",
    "timestamp": 1694908345000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1016/0014-5793%2890%2980704-M"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1990,
        4,
        9
      ]
    ]
  },
  "references-count": 14,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1990,
          4,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1016/0014-5793(90)80704-M"
  ],
  "URL": "http://dx.doi.org/10.1016/0014-5793(90)80704-M",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        1990,
        4,
        9
      ]
    ]
  }
}