DOI: 10.1016/0014-5793(90)80649-4. N5,N10‐Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity

N5,N10‐Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity

10.1016/0014-5793(90)80649-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

N5,N10‐Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum (strain Marburg) was purified under anaerobic conditions to apparent homogeneity and a specific activity of approximately 750 μol/min/mg protein. Polyacrylamide gel electrophoresis under native and denaturing conditions revealed that the enzyme is composed of only one polypeptide with an apparent molecular mass of 43 kDa. The purified enzyme catalyzed the dehydrogenation of N5,N10‐methylenetetrahydromethanopterin (CH2=H4MPT) (apparent Km≡20 μM) to N5,N10‐methenyltetrahydromethanopterin (CH≡H4MPT) in the absence of any added electron acceptors. One mol of H2 was generated per mol CH≡H4MPT formed, indicating that protons served as electron acceptor. Coenzyme F420, NAD, NADP and viologen dyes were not reduced by CH2=H4MPT. The dehydrogenase also catalyzed the reverse reaction, the reduction of CH≡H4MPT to CH2=H4MPT with H2. The data indicate that CH2=H4MPT dehydrogenase from M. thermoautotrophicum is a novel type of hydrogenase.

Bibliography

Zirngibl, C., Hedderich, R., & Thauer, R. K. (1990). N5,N10âMethylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity. FEBS Letters, 261(1), 112â116. Portico.

Authors 3

C. Zirngibl (first)
R. Hedderich (additional)
R.K. Thauer (additional)

References 19 Referenced 153

10.1016/S0021-9258(18)68417-0
{'key': 'e_1_2_1_3_1', 'first-page': '95', 'volume': '1', 'author': 'Keltjens J.T.', 'year': '1988', 'journal-title': 'BioFactors'} / BioFactors by Keltjens J.T. (1988)
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{'key': 'e_1_2_1_6_1', 'first-page': '55', 'volume': '12', 'author': 'Te Brömmelstroet B.W.', 'year': '1989', 'journal-title': 'Forum Mikrobiol.'} / Forum Mikrobiol. by Te Brömmelstroet B.W. (1989)
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{'key': 'e_1_2_1_8_1', 'author': 'Borner G.', 'year': '1988', 'journal-title': 'Diploma thesis'} / Diploma thesis by Borner G. (1988)
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{'key': 'e_1_2_1_18_1', 'first-page': '285', 'volume-title': 'Metal Ions in Biological Systems', 'author': 'Moura J.J.G.', 'year': '1978'} / Metal Ions in Biological Systems by Moura J.J.G. (1978)
10.1073/pnas.86.13.4932
10.1016/S0723-2020(86)80036-4

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 8:38 p.m.)
Indexed	1 month, 1 week ago (July 16, 2025, 8:12 a.m.)
Issued	35 years, 6 months ago (Feb. 12, 1990)
Published	35 years, 6 months ago (Feb. 12, 1990)
Published Online	23 years, 9 months ago (Oct. 29, 2001)
Published Print	35 years, 6 months ago (Feb. 12, 1990)

Funders 0

None

BibTeX

@article{Zirngibl_1990, title={N5,N10‐Methylenetetrahydromethanopterin dehydrogenase from Methanobacterium thermoautotrophicum has hydrogenase activity}, volume={261}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(90)80649-4}, DOI={10.1016/0014-5793(90)80649-4}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Zirngibl, C. and Hedderich, R. and Thauer, R.K.}, year={1990}, month=feb, pages={112–116} }

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