DOI: 10.1016/0014-5793(90)80575-4. Identification of a G<sub>s</sub>‐protein coupling domain to the β‐aderenoceptor using site‐specific synthetic peptides

Identification of a Gs‐protein coupling domain to the β‐aderenoceptor using site‐specific synthetic peptides

10.1016/0014-5793(90)80575-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Competition between Gs‐protein and the synthetic peptide, GSA 379‐394, derived from the carboxyl‐terminal region of the αs‐subunit, led to complete inhibition of receptor‐mediated adenylate cyclase activation in turkey erythrocyte membranes. Related peptides corresponding to the homologous carboxyl‐terminal region of αt‐,αil‐ or αo‐subunits did not interfere with β‐receptor‐Gs coupling. The direct coupling between Gs and adenylate cyclase was not influenced by any of these peptides. These results emphasize the important role of the carboxyl‐terminus of G‐protein α‐subunits for the specific recognition of their corresponding receptors and for signal transduction.

Bibliography

Palm, D., MÃ¼nch, G., Malek, D., Dees, C., & Hekman, M. (1990). Identification of a Gsâprotein coupling domain to the Î²âaderenoceptor using siteâspecific synthetic peptides. FEBS Letters, 261(2), 294â298. Portico.

Authors 5

Dieter Palm (first)
Gerald Münch (additional)
Daria Malek (additional)
Christian Dees (additional)
Mirko Hekman (additional)

References 19 Referenced 53

10.1016/0014-5793(89)81015-4
10.1096/fasebj.3.10.2546847
10.1073/pnas.83.23.8893
10.1016/S0021-9258(18)42983-3
10.1016/0014-5793(86)81336-9
10.1093/protein/1.1.47
10.1016/0014-5793(87)80486-6
10.1038/330758a0
10.1126/science.3136547
10.1016/S0021-9258(19)76547-8
10.1016/0092-8674(82)90202-1
10.1016/0092-8674(83)90391-4
{'key': 'e_1_2_1_14_1', 'first-page': '145', 'volume-title': 'Synthetic Polypeptides as Antigens', 'author': 'Van Regenmortel M.H.V.', 'year': '1988'} / Synthetic Polypeptides as Antigens by Van Regenmortel M.H.V. (1988)
Product manual 1988 Janssen Biotech
10.1016/S0021-9258(19)42662-8
10.1002/j.1460-2075.1986.tb04390.x
10.1016/0003-2697(74)90222-X
10.1016/0014-5793(89)80622-2
10.1021/bi00414a001

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 9:19 p.m.)
Indexed	1 year, 4 months ago (April 15, 2024, 8:10 p.m.)
Issued	35 years, 6 months ago (Feb. 26, 1990)
Published	35 years, 6 months ago (Feb. 26, 1990)
Published Online	23 years, 9 months ago (Nov. 14, 2001)
Published Print	35 years, 6 months ago (Feb. 26, 1990)

Funders 0

None

BibTeX

@article{Palm_1990, title={Identification of a Gs‐protein coupling domain to the β‐aderenoceptor using site‐specific synthetic peptides: Carboxyl terminus of Gsα is involved in coupling to β‐adrenoceptors}, volume={261}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(90)80575-4}, DOI={10.1016/0014-5793(90)80575-4}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Palm, Dieter and Münch, Gerald and Malek, Daria and Dees, Christian and Hekman, Mirko}, year={1990}, month=feb, pages={294–298} }

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