DOI: 10.1016/0014-5793(90)80384-u. Electron microscopy of the reconstituted complexes of the F<sub>1</sub>‐ATPase with various subunit constitution revealed the location of the γ subunit in the central cavity of the molecule

Electron microscopy of the reconstituted complexes of the F1‐ATPase with various subunit constitution revealed the location of the γ subunit in the central cavity of the molecule

10.1016/0014-5793(90)80384-u

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The subunit structure of the F1‐ATPase from the thermophilic bacterium PS3 was probed by comparing electron microscopic images of the subunit complexes reconstituted to contain different subunit compositions. The following structural features were observed. (1) The α3β3 complex has a hexagonal, apparently symmetrical arrangement of masses with a central cavity. (2) The projections of the α3β3δ complexes are similar to those of the α3β3 complexes. (3) In contrast, the α3β3λ complex has an additional mass in the centre which is similar to that found in the native enzyme (α3β3γδϵ). From these observations, it is concluded that the central mass in the F1‐ATPase is comprised mostly of the γ subunit.

Bibliography

Fujiyama, Y., Yokoyama, K., Yoshida, M., & Wakabayashi, T. (1990). Electron microscopy of the reconstituted complexes of the F1âATPase with various subunit constitution revealed the location of the Î³ subunit in the central cavity of the molecule. FEBS Letters, 271(1â2), 111â115. Portico.

Authors 4

Yuichiro Fujiyama (first)
Ken Yokoyama (additional)
Masasuke Yoshida (additional)
Takeyuki Wakabayashi (additional)

References 24 Referenced 7

10.1146/annurev.bi.58.070189.000551
10.1152/physrev.1988.68.1.177
10.1016/0304-4173(84)90003-X
10.1016/0022-2836(77)90140-1
{'key': 'e_1_2_1_6_1', 'first-page': '15', 'volume': '7', 'author': 'Wakabayashi T.', 'year': '1982', 'journal-title': 'Tokai J. Exp. Clin. Med. Supp.'} / Tokai J. Exp. Clin. Med. Supp. by Wakabayashi T. (1982)
10.1111/j.1432-1033.1983.tb07322.x
10.1002/j.1460-2075.1983.tb01599.x
10.1016/0014-5793(84)80144-1
10.1016/0005-2728(86)90071-X
10.1016/0005-2728(88)90044-8
10.1021/bi00437a030
10.1016/0022-2836(84)90408-X
10.1073/pnas.82.23.7874
10.1021/bi00437a031
10.1016/S0021-9258(17)40416-9
10.1073/pnas.86.17.6484
10.1016/0014-5793(89)80017-1
10.1093/oxfordjournals.jbchem.a122981
10.1016/0006-291X(87)90586-9
10.1016/0005-2728(88)90064-3
10.1016/S0021-9258(20)88260-X
{'key': 'e_1_2_1_23_1', 'first-page': '112', 'volume': '14', 'author': 'Fukami A.', 'year': '1965', 'journal-title': 'J. Electron Microsc.'} / J. Electron Microsc. by Fukami A. (1965)
10.1016/S0022-5320(79)90109-6
10.1016/0022-2836(69)90128-4

Dates

Type	When
Created	23 years ago (July 25, 2002, 3:44 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:06 a.m.)
Indexed	1 year, 9 months ago (Oct. 24, 2023, 2:37 a.m.)
Issued	34 years, 10 months ago (Oct. 1, 1990)
Published	34 years, 10 months ago (Oct. 1, 1990)
Published Online	23 years, 9 months ago (Nov. 12, 2001)
Published Print	34 years, 10 months ago (Oct. 1, 1990)

Funders 0

None

BibTeX

@article{Fujiyama_1990, title={Electron microscopy of the reconstituted complexes of the F1‐ATPase with various subunit constitution revealed the location of the γ subunit in the central cavity of the molecule}, volume={271}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(90)80384-u}, DOI={10.1016/0014-5793(90)80384-u}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Fujiyama, Yuichiro and Yokoyama, Ken and Yoshida, Masasuke and Wakabayashi, Takeyuki}, year={1990}, month=oct, pages={111–115} }

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