DOI: 10.1016/0014-5793(89)80885-3. Methylation of elongation factor EF‐Tu affects the rate of trypsin degradation and tRNA‐dependent GTP hydrolysis

Methylation of elongation factor EF‐Tu affects the rate of trypsin degradation and tRNA‐dependent GTP hydrolysis

10.1016/0014-5793(89)80885-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The role of methylation of elongation factor EF‐Tu from Escherichia coli was investigated. The methylated factor was obtained from cells harvested at the late stationary growth phase. Submethylated forms of the factor were obtained from either bacteria grown to the mid‐logarithmic phase or cells cultured in the presence of ethionine. When fully methylated EF‐Tu was treated in the presence of trypsin, it showed a greatly reduced rate of degradation compared with both types of undermethylated EF‐Tu factors. The methylation of EF‐Tu appeared not to affect the rate of GDP and GTP exchange. However, aminoacyl‐tRNA‐stimulated GTPase activity determined in the presence of kirromycin was greatly increased when the EF‐Tu was methylated.

Bibliography

Toledo, H., & Jerez, C. A. (1989). Methylation of elongation factor EFâTu affects the rate of trypsin degradation and tRNAâdependent GTP hydrolysis. FEBS Letters, 252(1â2), 37â41. Portico.

Authors 2

Héctor Toledo (first)
Carlos A. Jerez (additional)

References 26 Referenced 5

{'key': 'e_1_2_1_2_1', 'first-page': '437', 'volume-title': 'Nucleic Acid Research, Future Development', 'author': 'Kaziro Y.', 'year': '1983'} / Nucleic Acid Research, Future Development by Kaziro Y. (1983)
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10.1016/S0021-9258(19)86650-4
10.1016/0014-5793(79)80407-X
10.1093/oxfordjournals.jbchem.a132638
10.1016/0014-5793(83)80115-X
10.1016/0014-5793(83)80311-1
10.1128/MCB.5.5.1100
10.1073/pnas.79.11.3433
10.1002/j.1460-2075.1984.tb01937.x
10.1111/j.1432-1033.1986.tb10074.x
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10.1016/S0076-6879(79)60056-3
10.1038/227680a0
10.1073/pnas.71.5.2136
{'key': 'e_1_2_1_21_1', 'first-page': '51', 'volume-title': 'Ribosomes: Structure, Function and Genetics', 'author': 'Wittmann H.G.', 'year': '1980'} / Ribosomes: Structure, Function and Genetics by Wittmann H.G. (1980)
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10.1111/j.1432-1033.1980.tb04738.x
10.1073/pnas.79.16.4922

Dates

Type	When
Created	23 years ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:50 a.m.)
Indexed	1 year, 11 months ago (Sept. 17, 2023, 12:57 a.m.)
Issued	36 years, 1 month ago (July 1, 1989)
Published	36 years, 1 month ago (July 1, 1989)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	36 years, 1 month ago (July 1, 1989)

Funders 0

None

BibTeX

@article{Toledo_1989, title={Methylation of elongation factor EF‐Tu affects the rate of trypsin degradation and tRNA‐dependent GTP hydrolysis}, volume={252}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(89)80885-3}, DOI={10.1016/0014-5793(89)80885-3}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Toledo, Héctor and Jerez, Carlos A.}, year={1989}, month=jul, pages={37–41} }

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