DOI: 10.1016/0014-5793(89)80572-1. Immunoquantitation of 80 kDa diacylglycerol kinase in pig and human lymphocytes and several other cells

Immunoquantitation of 80 kDa diacylglycerol kinase in pig and human lymphocytes and several other cells

10.1016/0014-5793(89)80572-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

80 kDa diacylglycerol kinase (DGK) was immunoquantitated in cell homogenates and subcellular fractions. It was extremely abundant in the cytosol of various lymphocytes and comprised, in the highest case, more than 0.2% of the total soluble protein in T cell‐enriched pig splenocytes. The lymphocyte membrane contained less than 10% of the total cellular DGK protein. The content of 80 kDa DGK in the human T cell leukemic cell line, Jurkat (360 ng/mg homogenate protein), was similar to those in pig and human peripheral blood lymphocytes. In contrast, the enzyme level was very low in the human promyeloblastic cell line, HL‐60 (< 10 ng/mg homogenate protein), and was undetectable in human polymorphonuclear leukocytes. These findings indicate that the content of 80 kDa DGK is markedly variable depending on the type of cells, even though all these cells are known to accumulate phosphatidate rapidly upon cell stimulation.

Bibliography

Yamada, K., Sakane, F., & Kanoh, H. (1989). Immunoquantitation of 80 kDa diacylglycerol kinase in pig and human lymphocytes and several other cells. FEBS Letters, 244(2), 402â406. Portico.

Authors 3

Keiko Yamada (first)
Fumio Sakane (additional)
Hideo Kanoh (additional)

References 25 Referenced 41

10.1016/0304-4157(75)90017-9
10.1146/annurev.bi.54.070185.001225
10.1038/308693a0
10.1016/S0021-9258(19)62713-4
10.1016/S0021-9258(17)36324-X
10.1016/S0021-9258(18)33053-9
10.1016/S0021-9258(18)90846-X
10.1016/S0021-9258(19)57256-8
{'key': 'e_1_2_1_10_1', 'first-page': '601', 'volume': '255', 'author': 'Yamada K.', 'year': '1988', 'journal-title': 'Biochem. J.'} / Biochem. J. by Yamada K. (1988)
10.1016/S0021-9258(19)57343-4
10.1016/S0021-9258(19)57344-6
10.1016/S0021-9258(19)84554-4
10.1016/S0021-9258(18)66830-9
10.1016/S0006-291X(88)80477-7
{'key': 'e_1_2_1_16_1', 'first-page': '77', 'volume-title': 'Immunochemistry in Practice', 'author': 'Johnstone A.', 'year': '1982'} / Immunochemistry in Practice by Johnstone A. (1982)
10.1038/227680a0
10.1016/S0003-2697(76)80064-4
10.1111/j.1471-4159.1978.tb00126.x
10.1111/j.1432-1033.1978.tb12230.x
10.1007/BF02533542
10.1016/S0021-9258(17)44677-1
10.1073/pnas.83.24.9378
10.1016/S0006-291X(87)80066-9
10.1016/0006-291X(88)90494-9
{'key': 'e_1_2_1_26_1', 'author': 'Kanoh H.', 'year': '1988', 'journal-title': 'Biochem. J.'} / Biochem. J. by Kanoh H. (1988)

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:40 a.m.)
Indexed	2 days, 9 hours ago (Sept. 4, 2025, 9:55 a.m.)
Issued	36 years, 6 months ago (Feb. 27, 1989)
Published	36 years, 6 months ago (Feb. 27, 1989)
Published Online	23 years, 9 months ago (Nov. 14, 2001)
Published Print	36 years, 6 months ago (Feb. 27, 1989)

Funders 0

None

BibTeX

@article{Yamada_1989, title={Immunoquantitation of 80 kDa diacylglycerol kinase in pig and human lymphocytes and several other cells}, volume={244}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(89)80572-1}, DOI={10.1016/0014-5793(89)80572-1}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Yamada, Keiko and Sakane, Fumio and Kanoh, Hideo}, year={1989}, month=feb, pages={402–406} }

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