DOI: 10.1016/0014-5793(89)80516-2. SecA protein is directly involved in protein secretion in <i>Escherichia coli</i>

SecA protein is directly involved in protein secretion in Escherichia coli

10.1016/0014-5793(89)80516-2

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

A high‐expression plasmid for the secA gene was constructed. The SecA protein was then overproduced in E. coli and purified. The purified SecA stimulated the in vitro translocation of a model secretory protein into inverted membrane vesicles pretreated with 4 M urea. Membrane vesicles from a secAts mutant exhibited lower translocation activity, which was enhanced by SecA. These results indicate that SecA is directly involved in protein secretion across the cytoplasmic membrane.

Bibliography

Kawasaki, H., Matsuyama, S., Sasaki, S., Akita, M., & Mizushima, S. (1989). SecA protein is directly involved in protein secretion in Escherichia coli. FEBS Letters, 242(2), 431â434. Portico.

Authors 5

Hisashi Kawasaki (first)
Shin-ichi Matsuyama (additional)
Shoichi Sasaki (additional)
Mitsuru Akita (additional)
Shoji Mizushima (additional)

References 12 Referenced 56

10.1016/0092-8674(81)90184-7
10.1128/jb.170.8.3404-3414.1988
{'key': 'e_1_2_1_4_1', 'first-page': '264', 'author': 'Yamada H.', 'year': '1989', 'journal-title': 'J. Biol. Chem.'} / J. Biol. Chem. by Yamada H. (1989)
10.1016/S0021-9258(18)60725-2
10.1016/0022-2836(76)90119-4
10.1016/0076-6879(79)68018-7
10.1016/S0021-9258(18)61662-X
10.1016/0092-8674(87)90503-4
10.1128/jb.150.2.686-691.1982
10.1128/jb.158.3.1198-1201.1984
10.1271/bbb1961.52.983
10.1016/0092-8674(82)90037-X

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 17, 2023, 2:52 a.m.)
Indexed	1 year, 1 month ago (July 22, 2024, 4:27 p.m.)
Issued	36 years, 8 months ago (Jan. 2, 1989)
Published	36 years, 8 months ago (Jan. 2, 1989)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	36 years, 8 months ago (Jan. 2, 1989)

Funders 0

None

BibTeX

@article{Kawasaki_1989, title={SecA protein is directly involved in protein secretion in Escherichia coli}, volume={242}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(89)80516-2}, DOI={10.1016/0014-5793(89)80516-2}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Kawasaki, Hisashi and Matsuyama, Shin-ichi and Sasaki, Shoichi and Akita, Mitsuru and Mizushima, Shoji}, year={1989}, month=jan, pages={431–434} }

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