DOI: 10.1016/0014-5793(89)80454-5. Inhibition of target cell mitochondrial electron transfer by tumor necrosis factor

Inhibition of target cell mitochondrial electron transfer by tumor necrosis factor

10.1016/0014-5793(89)80454-5

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Using digitonin permeabilization to assay mitochondrial electron transfer, we have found that respiratory activity (succinoxidase and cytochrome oxidase) in three mouse fibroblast lines is completely eliminated by incubation with human recombinant tumor necrosis factor‐α (hrTNF). As with cytotoxicity, hrTNF‐induced mitochondrial dysfunction occurs in resistant cells upon inhibition of protein synthesis, whereas sensitive cells exhibit spontaneous respiratory inhibition. In C3HA cells, inhibition is detectable 1.5–2 h after hrTNF addition, preceding cell lysis by at least 5 h (as measured by dye exclusion), and is approximately coincidental with morphological changes we have previously reported for this cell line. LM cells also exhibit inhibition of electron transfer, coincidental with morphological changes. These results suggest that bioenergetic dysfunction may be involved in the cytotoxic mechanism of TNF.

Bibliography

Lancaster, J. R., Laster, S. M., & Gooding, L. R. (1989). Inhibition of target cell mitochondrial electron transfer by tumor necrosis factor. FEBS Letters, 248(1â2), 169â174. Portico.

Authors 3

Jack R. Lancaster (first)
Scott M. Laster (additional)
Linda R. Gooding (additional)

References 49 Referenced 116

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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 4:13 a.m.)
Indexed	2 months ago (July 2, 2025, 2:30 p.m.)
Issued	36 years, 3 months ago (May 8, 1989)
Published	36 years, 3 months ago (May 8, 1989)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	36 years, 3 months ago (May 8, 1989)

Funders 0

None

BibTeX

@article{Lancaster_1989, title={Inhibition of target cell mitochondrial electron transfer by tumor necrosis factor}, volume={248}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(89)80454-5}, DOI={10.1016/0014-5793(89)80454-5}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Lancaster, Jack R. and Laster, Scott M. and Gooding, Linda R.}, year={1989}, month=may, pages={169–174} }

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