DOI: 10.1016/0014-5793(88)80854-8. Protein immobilization on the surface of liposomes via carbodiimide activation in the presence of <i>N</i>‐hydroxysulfosuccinimide

Protein immobilization on the surface of liposomes via carbodiimide activation in the presence of N‐hydroxysulfosuccinimide

10.1016/0014-5793(88)80854-8

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

A method of the covalent immobilization of proteins on the surface of liposomes, containing 10% (by mol) of N‐glutaryl phosphatidylethanolamine, is described. Carboxylic groups of liposomal N‐glutaryl phosphatidylethanolamine were activated in the presence of water‐soluble carbodiimide and N‐hydroxysulfosuccinimide and reacted subsequently with protein amino groups. The liposome‐protein conjugates formed contained up to 5 × 10−4 mol protein/mol lipid. Lectins (RCAI and WGA) upon immobilization on liposomes retained saccharide specificity and the ability to agglutinate red blood cells. The immobilization of mouse monoclonal IgG in a ratio of 3.5 × 10−4 mol IgG/mol lipid was achieved. The liposome activation in the absence of N‐hydroxysulfosuccinimide resulted in a 2‐fold decrease of protein coupling yields.

Bibliography

Bogdanov, A. A., Klibanov, A. L., & Torchilin, V. P. (1988). Protein immobilization on the surface of liposomes via carbodiimide activation in the presence of Nâhydroxysulfosuccinimide. FEBS Letters, 231(2), 381â384. Portico.

Authors 3

A.A. Bogdanov (first)
A.L. Klibanov (additional)
V.P. Torchilin (additional)

References 21 Referenced 59

{'key': 'e_1_2_1_2_1', 'first-page': '65', 'volume': '2', 'author': 'Torchilin V.P.', 'year': '1985', 'journal-title': 'CRC Crit. Rev. Ther. Drug Carrier Systems'} / CRC Crit. Rev. Ther. Drug Carrier Systems by Torchilin V.P. (1985)
10.1016/0163-7258(85)90058-0
10.1016/S0021-9258(19)68359-6
10.1021/bi00517a043
10.1042/bj1730723
10.1016/0014-5793(87)80004-2
10.1016/0005-2736(85)90430-4
{'key': 'e_1_2_1_9_1', 'first-page': '674', 'volume': '3', 'author': 'Bogdanov A.A.', 'year': '1986', 'journal-title': 'Biol. Membr.'} / Biol. Membr. by Bogdanov A.A. (1986)
1988 Wiley London L.B. Margolis A.A. Bogdanov L.V. Gordeeva V.P. Torchilin G. Gregoriadis Liposomes in Drug Targeting: Trends and Progress 727 735
10.1021/bi00260a008
10.1016/0014-5793(86)80654-8
10.1073/pnas.75.9.4194
10.1016/0005-2736(80)90558-1
10.1021/bi00615a031
10.1016/0014-5793(82)80408-0
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10.1016/0005-2736(81)90532-0
10.1073/pnas.75.1.308
10.1016/0304-4157(84)90018-2

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 15, 2023, 9:03 p.m.)
Indexed	4 months, 3 weeks ago (April 17, 2025, 11:26 a.m.)
Issued	37 years, 4 months ago (April 25, 1988)
Published	37 years, 4 months ago (April 25, 1988)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	37 years, 4 months ago (April 25, 1988)

Funders 0

None

BibTeX

@article{Bogdanov_1988, title={Protein immobilization on the surface of liposomes via carbodiimide activation in the presence of N‐hydroxysulfosuccinimide}, volume={231}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(88)80854-8}, DOI={10.1016/0014-5793(88)80854-8}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Bogdanov, A.A. and Klibanov, A.L. and Torchilin, V.P.}, year={1988}, month=apr, pages={381–384} }

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