DOI: 10.1016/0014-5793(88)80589-1. Partial separation of inwardly pumping and outwardly pumping bacteriorhodopsin reconstituted liposomes by gel filtration

Partial separation of inwardly pumping and outwardly pumping bacteriorhodopsin reconstituted liposomes by gel filtration

10.1016/0014-5793(88)80589-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Reconstitution of bacteriorhodopsin using the reverse‐phase evaporation technique leads to the formation of both inwardly proton pumping and outwardly proton pumping liposomes [(1985) FEBS Lett. 188, 101–1061]. The reconstituted preparation was size fractionated using gel filtration chromatography on Sephacryl S1000. The direction of proton pumping was assessed in various fractions using the pH sensitive fluorescence probe pyranine. The proportion of outwardly pumping liposomes was shown to increase with decreasing liposome size. The larger liposomes (diameter larger than 200 nm) were shown to be pure inwardly pumping liposomes with almost homogeneous bacteriorhodopsin orientation.

Bibliography

Seigneuret, M., & Rigaud, J.-L. (1988). Partial separation of inwardly pumping and outwardly pumping bacteriorhodopsin reconstituted liposomes by gel filtration. FEBS Letters, 228(1), 79â84. Portico.

Authors 2

Michel Seigneuret (first)
Jean-Louis Rigaud (additional)

References 18 Referenced 21

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 9:09 a.m.)
Indexed	1 year ago (Aug. 15, 2024, 8:32 a.m.)
Issued	37 years, 6 months ago (Feb. 8, 1988)
Published	37 years, 6 months ago (Feb. 8, 1988)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	37 years, 6 months ago (Feb. 8, 1988)

Funders 0

None

BibTeX

@article{Seigneuret_1988, title={Partial separation of inwardly pumping and outwardly pumping bacteriorhodopsin reconstituted liposomes by gel filtration}, volume={228}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(88)80589-1}, DOI={10.1016/0014-5793(88)80589-1}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Seigneuret, Michel and Rigaud, Jean-Louis}, year={1988}, month=feb, pages={79–84} }

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