DOI: 10.1016/0014-5793(88)80369-7. Mobile sequences in the pyruvate dehydrogenase complex, the E2 component, the catalytic domain and the 2‐oxoglutarate dehydrogenase complex of Azotobacter vinelandii, as detected by 600 MHz 1H‐NMR spectroscopy

Mobile sequences in the pyruvate dehydrogenase complex, the E2 component, the catalytic domain and the 2‐oxoglutarate dehydrogenase complex of Azotobacter vinelandii, as detected by 600 MHz 1H‐NMR spectroscopy

10.1016/0014-5793(88)80369-7

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

600 MHz 1H‐NMR spectroscopy demonstrates that the pyruvate dehydrogenase complex of Azotobacter vinelandii contains regions of the polypeptide chain with intramolecular mobility. This mobility is located in the E2 component and can probably be ascribed to alanine‐proline‐rich regions that link the lipoyl subdomains to each other as well as to the E1 and E3 binding domain. In the catalytic domain of E2, which is thought to form a compact, rigid core, also conformational flexibility is observed. It is conceivable that the N‐terminal region of the catalytic domain, which contains many alanine residues, is responsible for the observed mobility. In the low‐field region of the 1H‐NMR spectrum of E2 specific resonances are found, which can be ascribed to mobile phenylalanine, histidine and/or tyrosine residues which are located in the E1 and E3 binding domain that links the lipoyl domain to the catalytic domain. In the 1H‐NMR spectrum of the intact complex, these resonances cannot be observed, indicating a decreased mobility of the E1 and E3 binding domain.

Bibliography

Hanemaaijer, R., Vervoort, J., Westphal, A. H., de Kok, A., & Veeger, C. (1988). Mobile sequences in the pyruvate dehydrogenase complex, the E2 component, the catalytic domain and the 2âoxoglutarate dehydrogenase complex of Azotobacter vinelandii, as detected by 600 MHz 1HâNMR spectroscopy. FEBS Letters, 240(1â2), 205â210. Portico.

Authors 5

Roeland Hanemaaijer (first)
Jacques Vervoort (additional)
Adrie H. Westphal (additional)
Arie de Kok (additional)
Cees Veeger (additional)

References 24 Referenced 6

10.1016/S0021-9258(19)83957-1
10.1111/j.1432-1033.1987.tb13604.x
Hanemaaijer R. Westphal A.H. Van de Heiden T. De Kok A. and Veeger C. Eur. J. Biochem. submitted.
10.1111/j.1432-1033.1988.tb14140.x
10.1002/j.1460-2075.1984.tb01969.x
10.1111/j.1432-1033.1983.tb07490.x
10.1021/bi00643a012
10.1111/j.1432-1033.1978.tb12136.x
10.1038/292474a0
{'key': 'e_1_2_1_11_1', 'first-page': '47', 'volume': '93', 'author': 'Roberts G.C.K.', 'year': '1983', 'journal-title': 'Ciba Symp.'} / Ciba Symp. by Roberts G.C.K. (1983)
10.1111/j.1432-1033.1982.tb05906.x
10.1042/bj2170219
10.1016/0014-5793(81)80908-8
10.1042/bj2470641
10.1021/bi00401a044
{'key': 'e_1_2_1_17_1', 'author': 'Hanemaaijer R.', 'year': '1988', 'journal-title': 'FEBS Lett.'} / FEBS Lett. by Hanemaaijer R. (1988)
Hanemaaijer R. Westphal A.H. Berg A. De Kok A. and Veeger C. Eur. J. Biochem. submitted.
10.1111/j.1749-6632.1982.tb31202.x
{'key': 'e_1_2_1_20_1', 'author': 'Bosma H.J.', 'year': '1984', 'journal-title': 'Ph.D. Thesis'} / Ph.D. Thesis by Bosma H.J. (1984)
10.1016/S0021-9258(19)52451-6
10.1042/bst0141231
10.1111/j.1432-1033.1980.tb04582.x
10.1042/bj2520079
10.1051/epn/19861701011

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 6:22 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 4:39 p.m.)
Issued	36 years, 9 months ago (Nov. 21, 1988)
Published	36 years, 9 months ago (Nov. 21, 1988)
Published Online	23 years, 10 months ago (Oct. 29, 2001)
Published Print	36 years, 9 months ago (Nov. 21, 1988)

Funders 0

None

BibTeX

@article{Hanemaaijer_1988, title={Mobile sequences in the pyruvate dehydrogenase complex, the E2 component, the catalytic domain and the 2‐oxoglutarate dehydrogenase complex of Azotobacter vinelandii, as detected by 600 MHz 1H‐NMR spectroscopy}, volume={240}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(88)80369-7}, DOI={10.1016/0014-5793(88)80369-7}, number={1–2}, journal={FEBS Letters}, publisher={Wiley}, author={Hanemaaijer, Roeland and Vervoort, Jacques and Westphal, Adrie H. and de Kok, Arie and Veeger, Cees}, year={1988}, month=nov, pages={205–210} }

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