DOI: 10.1016/0014-5793(87)81433-3. Correlation among sites of limited proteolysis, enzyme accessibility and segmental mobility

Correlation among sites of limited proteolysis, enzyme accessibility and segmental mobility

10.1016/0014-5793(87)81433-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The relationship among accessibility to an enzyme, flexibility, and limited proteolysis was explored. Regions accessible to large probes, comparable in size to proteolytic enzymes, were computed in the crystallographic structures of thermolysin, trypsinogen and ribonuclease. Positions of these accessible regions were compared with sites of autolytic/proteolytic attacks, and with locations of flexible backbone segments. All the proteolytic sites were found to be exceptionally accessible. Most of them were also flexible, but at least one prominent site in trypsinogen appeared to be rigid. Thus, surface exposure seems to be more essential to proteolysis than flexibility.

Bibliography

NovotnÃ½, J., & Bruccoleri, R. E. (1987). Correlation among sites of limited proteolysis, enzyme accessibility and segmental mobility. FEBS Letters, 211(2), 185â189. Portico.

Authors 2

Jiří Novotný (first)
Robert E. Bruccoleri (additional)

References 23 Referenced 59

10.1021/bi00356a001
{'key': 'e_1_2_1_3_1', 'first-page': '501', 'volume-title': 'Protein Folding', 'author': 'Neurath H.', 'year': '1980'} / Protein Folding by Neurath H. (1980)
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Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:37 p.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 2:30 p.m.)
Issued	38 years, 7 months ago (Jan. 26, 1987)
Published	38 years, 7 months ago (Jan. 26, 1987)
Published Online	23 years, 10 months ago (Oct. 29, 2001)
Published Print	38 years, 7 months ago (Jan. 26, 1987)

Funders 0

None

BibTeX

@article{Novotn__1987, title={Correlation among sites of limited proteolysis, enzyme accessibility and segmental mobility}, volume={211}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(87)81433-3}, DOI={10.1016/0014-5793(87)81433-3}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Novotný, Jiří and Bruccoleri, Robert E.}, year={1987}, month=jan, pages={185–189} }

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