DOI: 10.1016/0014-5793(87)80966-3. Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type‐2A protein phosphatase

Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type‐2A protein phosphatase

10.1016/0014-5793(87)80966-3

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

A 2.5 kb clone containing the full‐length coding sequence of a type‐2A protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The sequence of the protein deduced from the cDNA contains 309 residues (35.58 kDa). A major mRNA species at 2.0 kb and a minor component at 2.8 kb were visualized by Northern blotting in both skeletal muscle and liver. The type‐2A enzyme showed weak homology with mammalian alkaline phosphatases between residues 55 and 95. The protein sequence of the type‐2A phosphatase from rabbit skeletal muscle differs from that reported for the bovine adrenal enzyme in three regions.

Bibliography

da Cruz e Silva, O. B., Alemany, S., Campbell, D. G., & Cohen, P. T. W. (1987). Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a typeâ2A protein phosphatase. FEBS Letters, 221(2), 415â422. Portico.

Authors 4

Odete B. da Cruz e Silva (first)
Susana Alemany (additional)
David G. Campbell (additional)
Patricia T.W. Cohen (additional)

References 32 Referenced 72

10.1111/j.1432-1033.1985.tb09121.x
10.1126/science.6306765
10.1111/j.1432-1033.1983.tb07357.x
1986 Leuven University Press Belgium A.P. Bradford S.J. Yeaman W. Merlevede J. DiSalvo Advances in Protein Phosphatases III 73 106
{'key': 'e_1_2_1_6_1', 'first-page': '163', 'volume': '107', 'author': 'Foulkes J.G.', 'year': '1983', 'journal-title': 'Curr. Top. Microbiol. Immunol.'} / Curr. Top. Microbiol. Immunol. by Foulkes J.G. (1983)
10.1111/j.1432-1033.1983.tb07362.x
{'key': 'e_1_2_1_8_1', 'author': 'Cohen P.T.W.', 'year': '1987', 'journal-title': '1st International Meeting on Post-translational Modifications and Ageing, Ischia, Italy, May 1987'} / 1st International Meeting on Post-translational Modifications and Ageing, Ischia, Italy, May 1987 by Cohen P.T.W. (1987)
10.1111/j.1432-1033.1983.tb07485.x
10.1111/j.1432-1033.1984.tb07962.x
10.1111/j.1432-1033.1986.tb09473.x
10.1016/0022-2836(85)90276-1
10.1093/nar/11.8.2325
10.1073/pnas.82.7.1931
10.1016/0014-5793(87)80871-2
1986 Cold Spring Harbor Laboratory NY R.W. Davis D. Botstein J.R. Roth Advances in Bacterial Genetics: A Manual for Genetic Engineering
1986 Stratagene San Diego USA Bluescript Exo/Mung DNA Sequencing System
1982 Cold Spring Harbor Laboratory NY T. Maniatis E.F. Fritsch J. Sambrook Molecular Cloning: A Laboratory Handbook
10.1016/0003-2697(81)90473-5
10.1089/dna.1985.4.165
10.1073/pnas.74.12.5463
1984 Amersham International Bucks England M13 Cloning and Sequencing Handbook
10.1111/j.1432-1033.1985.tb08833.x
10.1111/j.1432-1033.1985.tb09227.x
10.1073/pnas.83.21.8132
{'key': 'e_1_2_1_26_1', 'author': 'McGowan C.H.', 'year': '1987', 'journal-title': 'Eur. J. Biochem.'} / Eur. J. Biochem. by McGowan C.H. (1987)
10.1073/pnas.84.14.4880
10.1073/pnas.82.24.8715
10.1016/S0021-9258(17)35755-1
10.1073/pnas.83.19.7182
10.1073/pnas.84.3.695
10.1073/pnas.84.5.1234
10.1093/nar/9.21.5671

Dates

Type	When
Created	23 years ago (July 25, 2002, 3:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 11:43 p.m.)
Indexed	2 weeks, 4 days ago (Aug. 5, 2025, 8:40 a.m.)
Issued	37 years, 11 months ago (Sept. 14, 1987)
Published	37 years, 11 months ago (Sept. 14, 1987)
Published Online	23 years, 10 months ago (Oct. 19, 2001)
Published Print	37 years, 11 months ago (Sept. 14, 1987)

Funders 0

None

BibTeX

@article{da_Cruz_e_Silva_1987, title={Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type‐2A protein phosphatase}, volume={221}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(87)80966-3}, DOI={10.1016/0014-5793(87)80966-3}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={da Cruz e Silva, Odete B. and Alemany, Susana and Campbell, David G. and Cohen, Patricia T.W.}, year={1987}, month=sep, pages={415–422} }

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