DOI: 10.1016/0014-5793(86)80473-2. Stable binding interactions among subunits of the chloroplast ATP synthase (CF<sub>1</sub>‐CF<sub>0</sub> as examined by solid support (nitrocellulose)‐subunit reconstitution‐immunoblotting

Stable binding interactions among subunits of the chloroplast ATP synthase (CF1‐CF0 as examined by solid support (nitrocellulose)‐subunit reconstitution‐immunoblotting

10.1016/0014-5793(86)80473-2

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The technique termed solid support‐subunit reconstitution‐immunoblotting has been applied to examine stable binding interactions among renatured subunits of the chloroplast ATP synthase. Reconstitution of the CF1 subunits α, β and γ and subunit III of CF0 with thylakoid membrane polypeptides separated electrophoretically and transferred to nitrocellulose filters followed by immunodetection of hybrid subunit complexes revealed interactions between α‐ϵ, α‐CF0‐I, α‐CF0‐II, α‐δ, β‐γ, β‐CF0‐II β‐ϵ, β‐CF0‐III, γ‐CF0‐II, γ‐ϵ, γ‐CF0‐III, CF0‐III‐ϵ. Thus, the data confirm results on neighbour relationships of ATP synthase subunits obtained by cross‐linking and suggest that the method employed is generally applicable to examine protein‐protein binding interactions.

Bibliography

SÃ¼ss, K.-H. (1986). Stable binding interactions among subunits of the chloroplast ATP synthase (CF1âCF0 as examined by solid support (nitrocellulose)âsubunit reconstitutionâimmunoblotting. FEBS Letters, 199(2), 169â172. Portico.

Authors 1

Karl-Heinz Süss (first)

References 17 Referenced 7

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10.1016/0304-4173(84)90005-3
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10.1002/j.1460-2075.1985.tb03790.x
10.1016/S0021-9258(17)43905-6
10.1016/S0021-9258(17)43898-1
{'key': 'e_1_2_1_12_1', 'volume': '200', 'author': 'Süss K.-H.', 'year': '1986', 'journal-title': 'FEBS Lett.'} / FEBS Lett. by Süss K.-H. (1986)
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{'key': 'e_1_2_1_16_1', 'first-page': '59', 'volume-title': 'Transport and Bioenergetics in Biomembranes', 'author': 'Futai M.', 'year': '1982'} / Transport and Bioenergetics in Biomembranes by Futai M. (1982)
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10.1016/S0015-3796(82)80018-8

Dates

Type	When
Created	23 years ago (July 25, 2002, 3:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 2:25 a.m.)
Indexed	1 year, 11 months ago (Sept. 17, 2023, 12:56 a.m.)
Issued	39 years, 4 months ago (April 21, 1986)
Published	39 years, 4 months ago (April 21, 1986)
Published Online	23 years, 8 months ago (Dec. 5, 2001)
Published Print	39 years, 4 months ago (April 21, 1986)

Funders 0

None

BibTeX

@article{S_ss_1986, title={Stable binding interactions among subunits of the chloroplast ATP synthase (CF1‐CF0 as examined by solid support (nitrocellulose)‐subunit reconstitution‐immunoblotting}, volume={199}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(86)80473-2}, DOI={10.1016/0014-5793(86)80473-2}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Süss, Karl-Heinz}, year={1986}, month=apr, pages={169–172} }

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