DOI: 10.1016/0014-5793(86)80060-6. A calcium‐protease activator associated with brain microsomal‐insoluble elements

A calcium‐protease activator associated with brain microsomal‐insoluble elements

10.1016/0014-5793(86)80060-6

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

A factor which markedly activates Ca2+‐dependent thiol protease (calpain) is associated with Triton X‐100‐insoluble materials, presumably structural elements such as cytoskeletons, of bovine brain microsomal fraction. This factor is extracted with 0.6 M KCl, and purified partially by sucrose density gradient centrifugation and hydroxyapatite column chromatography. The factor appears to be a heat‐stable protein with an approximate M r, of 15000. With casein as substrate this factor activates both calpain I and calpain II severalfold up to more than 10‐ fold without alteration of their affinity to Ca2+. Calmodulin is unable to substitute for this factor. A similar factor is associated with human platelet insoluble materials.

Bibliography

Takeyama, Y., Nakanishi, H., Uratsuji, Y., Kishimoto, A., & Nishizuka, Y. (1986). A calciumâprotease activator associated with brain microsomalâinsoluble elements. FEBS Letters, 194(1), 110â114. Portico.

Authors 5

Yoshihumi Takeyama (first)
Hiroyuki Nakanishi (additional)
Yuko Uratsuji (additional)
Akira Kishimoto (additional)
Yasutomi Nishizuka (additional)

References 25 Referenced 38

Dates

Type	When
Created	23 years ago (July 25, 2002, 3:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 12:58 p.m.)
Indexed	1 year, 7 months ago (Dec. 23, 2023, 5:15 p.m.)
Issued	39 years, 7 months ago (Jan. 1, 1986)
Published	39 years, 7 months ago (Jan. 1, 1986)
Published Online	23 years, 9 months ago (Oct. 29, 2001)
Published Print	39 years, 7 months ago (Jan. 1, 1986)

Funders 0

None

BibTeX

@article{Takeyama_1986, title={A calcium‐protease activator associated with brain microsomal‐insoluble elements}, volume={194}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(86)80060-6}, DOI={10.1016/0014-5793(86)80060-6}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Takeyama, Yoshihumi and Nakanishi, Hiroyuki and Uratsuji, Yuko and Kishimoto, Akira and Nishizuka, Yasutomi}, year={1986}, month=jan, pages={110–114} }

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