DOI: 10.1016/0014-5793(86)80032-1. Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl‐tRNA

Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl‐tRNA

10.1016/0014-5793(86)80032-1

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Suppressor [32P]phosphoseryl‐tRNA, prepared using bovine seryl‐tRNA synthetase and ATP:seryl‐tRNA phosphotransferase, was mixed with rabbit reticulocyte lysates containing endogenous hemoglobin mRNA having the termination codon UGA (opal). The Chromatographic pattern of the lysate on Sephacryl S‐200 showed that the radioactivity of [32P]Pjphosphate in the hot trichloroacetic acid‐precipitate (phosphoprotein) was eluted at the position between mature hemoglobin and globin subunits. The phosphoprotein, obtained by chromatography on S‐200, moved to the position corresponding to that of globin readthrough protein on SDS‐PAGE. The analyses of the hydrolyzate of the phosphoprotein showed the presence of phosphoserine in the protein. These results suggest that animal opal suppressor tRNA functions in vitro to transfer phosphoserine to the position of the termination codon UGA (opal) on mRNA.

Bibliography

Mizutani, T., & Tachibana, Y. (1986). Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoserylâtRNA. FEBS Letters, 207(1), 162â166. Portico.

Authors 2

Takaharu Mizutani (first)
Yoshio Tachibana (additional)

References 25 Referenced 19

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 3:39 p.m.)
Indexed	3 months, 1 week ago (May 22, 2025, 5:47 a.m.)
Issued	38 years, 10 months ago (Oct. 20, 1986)
Published	38 years, 10 months ago (Oct. 20, 1986)
Published Online	23 years, 9 months ago (Nov. 12, 2001)
Published Print	38 years, 10 months ago (Oct. 20, 1986)

Funders 0

None

BibTeX

@article{Mizutani_1986, title={Possible incorporation of phosphoserine into globin readthrough protein via bovine opal suppressor phosphoseryl‐tRNA}, volume={207}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(86)80032-1}, DOI={10.1016/0014-5793(86)80032-1}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Mizutani, Takaharu and Tachibana, Yoshio}, year={1986}, month=oct, pages={162–166} }

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