DOI: 10.1016/0014-5793(86)80019-9. Exogenous ATP raises cytoplasmic free calcium in fura‐2 loaded piglet aortic endothelial cells

Exogenous ATP raises cytoplasmic free calcium in fura‐2 loaded piglet aortic endothelial cells

10.1016/0014-5793(86)80019-9

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Cultured piglet endothelial cells were grown to confluence on glass coverslips and loaded with the fluorescent Ca2+ indicator, fura‐2. Using a dual‐wavelength excitation fluorescence spectrophotometer it was found that ATP caused a rapid transient elevation in [Ca2+]i in the presence of extracellular calcium which decreased to a maintained elevated level. With no extracellular calcium ATP evoked a similar transient increase which returned to the basal level. Addition of 50 mM K+ had no effect on [Ca2+]i or on the effect of ATP on [Ca2+]i in the presence of extracellular Ca2+. The data suggest that ATP causes both discharge of calcium from an intracellular pool and influx across the plasma membrane although this is unlikely to be via a voltage‐operated channel. ATP stimulated simultaneously the production of PGI2 to a similar extent in the presence or absence of extracellular calcium. Elevated [Ca2+]i may be an important activation pathway in the endothelial cell.

Bibliography

Hallam, T. J., & Pearson, J. D. (1986). Exogenous ATP raises cytoplasmic free calcium in furaâ2 loaded piglet aortic endothelial cells. FEBS Letters, 207(1), 95â99. Portico.

Authors 2

Trevor J. Hallam (first)
Jeremy D. Pearson (additional)

References 17 Referenced 132

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:57 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 3:39 p.m.)
Indexed	3 days, 2 hours ago (Sept. 4, 2025, 9:40 a.m.)
Issued	38 years, 10 months ago (Oct. 20, 1986)
Published	38 years, 10 months ago (Oct. 20, 1986)
Published Online	23 years, 9 months ago (Nov. 12, 2001)
Published Print	38 years, 10 months ago (Oct. 20, 1986)

Funders 0

None

BibTeX

@article{Hallam_1986, title={Exogenous ATP raises cytoplasmic free calcium in fura‐2 loaded piglet aortic endothelial cells}, volume={207}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(86)80019-9}, DOI={10.1016/0014-5793(86)80019-9}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Hallam, Trevor J. and Pearson, Jeremy D.}, year={1986}, month=oct, pages={95–99} }

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