DOI: 10.1016/0014-5793(85)80223-4. Magnetic circular dichroism of DCPIP‐oxidised <i>Desulfovibrio vulgaris</i> hydrogenase

Magnetic circular dichroism of DCPIP‐oxidised Desulfovibrio vulgaris hydrogenase

10.1016/0014-5793(85)80223-4

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Ni‐free hydrogenases commonly exhibit a gav > 2 EPR spectrum in their most oxidised level. This spectrum has been linked with the active site and generally attributed to an oxidised HIPIP‐type, [4Fe‐4S]3+ cluster. We report the low temperature magnetic circular dichroism (MCD) spectrum of 2,6‐dichlorophenolindo‐phenol (DCPIP)‐oxidised Desulfovibrio vulgaris hydrogenase (DvH2ase), which exhibits an axial gav > 2 EPR spectrum. Paramagnetic MCD is observed, which however is shown to arise from an EPR‐silent, para‐magnetic species with S > . No evidence of paramagnetic MCD arising from the gav > 2 EPR‐detectable species is obtained. We conclude that: (1) DCPIP‐oxidised DvH2ase does not contain either a [4Fe‐4S]3+ or an oxidised 3Fe cluster; (ii) an EPR‐silent species with MCD characteristics somewhat similar to those of the oxidised ‘P’ clusters of the dye‐oxidized iron‐molybdenum protein of nitrogenase is present in DCPIP‐oxidised DvH2ase; (iii) the MCD and EPR of the gav > 2 species exhibit characteristics in common with those of the gav > 2 species produced by Fe(CN)6 3− oxidation of the [4Fe‐4S]2+ cluster of the 7Fe ferredoxin I of Azotobacter vinelandii [(7Fe)FdI). We suggest that the gav > 2 EPR signal of this and other hydrogenases arises from a species chemically analogous to that observed in (7Fe)FdI.

Bibliography

Stephens, P. J., Devlin, F., McKenna, M. C., Morgan, T. V., Czechowski, M., DerVartanian, D. V., Peck, H. D., & LeGall, J. (1985). Magnetic circular dichroism of DCPIPâoxidised Desulfovibrio vulgaris hydrogenase. FEBS Letters, 180(1), 24â28. Portico.

Authors 8

P.J. Stephens (first)
F. Devlin (additional)
M.C. McKenna (additional)
T.V. Morgan (additional)
M. Czechowski (additional)
D.V. DerVartanian (additional)
H.D. Peck (additional)
J. LeGall (additional)

References 19 Referenced 20

10.1016/0304-4173(80)90007-5
10.1016/S0021-9258(17)39835-6
10.1016/0005-2795(74)90025-7
10.1021/ja00444a044
10.1007/978-1-4684-3270-1_6
10.1016/0003-9861(83)90531-3
10.1073/pnas.81.7.1931
10.1073/pnas.81.12.3728
10.1016/S0021-9258(17)40446-7
{'key': 'e_1_2_1_11_1', 'author': 'Devlin F.', 'year': '1984', 'journal-title': 'Rev. Sci. Inst.'} / Rev. Sci. Inst. by Devlin F. (1984)
10.1021/bi00615a026
10.1042/bj1910411
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{'key': 'e_1_2_1_15_1', 'author': 'Stephens P.J.', 'year': '1985', 'journal-title': 'Biochemistry'} / Biochemistry by Stephens P.J. (1985)
10.1016/0005-2795(81)90053-2
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10.1016/0005-2795(81)90094-5
10.1111/j.1432-1033.1983.tb07727.x

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:58 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 2:09 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 7:20 a.m.)
Issued	40 years, 7 months ago (Jan. 21, 1985)
Published	40 years, 7 months ago (Jan. 21, 1985)
Published Online	23 years, 9 months ago (Nov. 2, 2001)
Published Print	40 years, 7 months ago (Jan. 21, 1985)

Funders 0

None

BibTeX

@article{Stephens_1985, title={Magnetic circular dichroism of DCPIP‐oxidised Desulfovibrio vulgaris hydrogenase}, volume={180}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(85)80223-4}, DOI={10.1016/0014-5793(85)80223-4}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Stephens, P.J. and Devlin, F. and McKenna, M.C. and Morgan, T.V. and Czechowski, M. and DerVartanian, D.V. and Peck, H.D. and LeGall, J.}, year={1985}, month=jan, pages={24–28} }

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