DOI: 10.1016/0014-5793(84)80481-0. <sup>31</sup>P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes

31P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes

10.1016/0014-5793(84)80481-0

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the narrow resonance signal characterizing phospolipids inserted into a mixed micelle. Up to 0.25 g detergent/g protein could be incorporated into the membrane without solubilization. Higher detergent concentrations of up to 1.5–2 g detergent/g protein led to gradual solubilization. Although the method allows us to monitor the extent of solubilization of individual phospholipid classes, there was no evidence of either preferential solubilization or retention of a specific class of phospholipids.

Bibliography

Roux, M., & Champeil, P. (1984). 31P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes. FEBS Letters, 171(2), 169â172. Portico.

Authors 2

Michel Roux (first)
Philippe Champeil (additional)

References 13 Referenced 14

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:59 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 7:04 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 8:41 a.m.)
Issued	41 years, 2 months ago (June 11, 1984)
Published	41 years, 2 months ago (June 11, 1984)
Published Online	23 years, 9 months ago (Nov. 12, 2001)
Published Print	41 years, 2 months ago (June 11, 1984)

Funders 0

None

BibTeX

@article{Roux_1984, title={31P NMR as a tool for monitoring detergent solubilization of sarcoplasmic reticulum membranes}, volume={171}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(84)80481-0}, DOI={10.1016/0014-5793(84)80481-0}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Roux, Michel and Champeil, Philippe}, year={1984}, month=jun, pages={169–172} }

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