DOI: 10.1016/0014-5793(84)80294-x. Pore‐forming activity in the outer membrane of the chloroplast envelope

Pore‐forming activity in the outer membrane of the chloroplast envelope

10.1016/0014-5793(84)80294-x

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

The permeability properties of the outer membrane of the chloroplast envelope were studied. The exclusion limit for the penetration of molecules through this membrane into the intermembrane space lies between M r values of about 7000–13 000. This is the largest exclusion limit measured so far for pores of the porin type. In addition, the pore‐forming activity of the outer membrane of the chloroplast envelope was measured by reconstitution experiments. The pore has a single channel conductance of 0.72 nS, which is a linear function of the salt solution in the aqueous phase. The diameter of the chloroplast porin was calculated to be 2.5–3 nm, which is the largest diameter of all known porin pores, e.g., from mitochondria or Gram‐negative bacteria.

Bibliography

FlÃ¼gge, U. I., & Benz, R. (1984). Poreâforming activity in the outer membrane of the chloroplast envelope. FEBS Letters, 169(1), 85â89. Portico.

Authors 2

U.Ingo Flügge (first)
Roland Benz (additional)

References 28 Referenced 106

10.1126/science.202030
10.1016/0005-2728(71)90133-2
{'key': 'e_1_2_1_4_1', 'first-page': '215', 'volume-title': 'The Intact Chloroplast', 'author': 'Heldt H.W.', 'year': '1976'} / The Intact Chloroplast by Heldt H.W. (1976)
10.1104/pp.70.4.1032
10.1104/pp.60.2.286
10.1016/S0065-2911(08)60208-8
{'key': 'e_1_2_1_8_1', 'first-page': '123', 'volume-title': 'Transport in Biomembranes: Model Systems and Reconstitution', 'author': 'Benz R.', 'year': '1982'} / Transport in Biomembranes: Model Systems and Reconstitution by Benz R. (1982)
10.1016/S0021-9258(19)85942-2
10.1111/j.1432-1033.1982.tb06578.x
10.1016/0005-2736(82)90114-6
10.1016/0005-2728(74)90174-1
{'key': 'e_1_2_1_13_1', 'first-page': '153', 'volume-title': 'Biochemistry and Metabolism of Plant Lipids', 'author': 'Dorne J.', 'year': '1982'} / Biochemistry and Metabolism of Plant Lipids by Dorne J. (1982)
{'key': 'e_1_2_1_14_1', 'first-page': '203', 'author': 'Block M.A.', 'year': '1983', 'journal-title': 'Sixth International Congress on Photosynthesis'} / Sixth International Congress on Photosynthesis by Block M.A. (1983)
10.1016/0003-2697(78)90706-6
10.1016/0076-6879(67)10111-0
10.1016/0014-5793(70)80140-5
10.1016/0005-2736(78)90269-9
10.1016/0005-2736(89)90002-3
10.1016/0006-291X(76)90913-X
10.1016/0005-2736(79)90373-0
10.1016/S0021-9258(17)37790-6
10.1085/jgp.77.2.121
{'key': 'e_1_2_1_24_1', 'first-page': '225', 'volume': '38', 'author': 'Renkin E.M.', 'year': '1954', 'journal-title': 'J. Gen. Physiol.'} / J. Gen. Physiol. by Renkin E.M. (1954)
10.1016/0005-2736(75)90105-4
10.1016/0005-2736(81)90336-9
10.1016/0005-2728(81)90240-1
{'key': 'e_1_2_1_28_1', 'author': 'Benz R.', 'year': '1984', 'journal-title': 'Eur. J. Biochem.'} / Eur. J. Biochem. by Benz R. (1984)
{'key': 'e_1_2_1_29_1', 'first-page': '315', 'volume': '44', 'author': 'Carde J.P.', 'year': '1982', 'journal-title': 'Biol. Cell.'} / Biol. Cell. by Carde J.P. (1982)

Dates

Type	When
Created	23 years, 1 month ago (July 25, 2002, 3:59 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 4:17 a.m.)
Indexed	2 weeks, 5 days ago (Aug. 6, 2025, 8:39 a.m.)
Issued	41 years, 4 months ago (April 9, 1984)
Published	41 years, 4 months ago (April 9, 1984)
Published Online	23 years, 9 months ago (Nov. 2, 2001)
Published Print	41 years, 4 months ago (April 9, 1984)

Funders 0

None

BibTeX

@article{Fl_gge_1984, title={Pore‐forming activity in the outer membrane of the chloroplast envelope}, volume={169}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(84)80294-x}, DOI={10.1016/0014-5793(84)80294-x}, number={1}, journal={FEBS Letters}, publisher={Wiley}, author={Flügge, U.Ingo and Benz, Roland}, year={1984}, month=apr, pages={85–89} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        6
      ]
    ],
    "date-time": "2025-08-06T12:39:53Z",
    "timestamp": 1754483993319
  },
  "reference-count": 28,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            11,
            2
          ]
        ],
        "date-time": "2001-11-02T00:00:00Z",
        "timestamp": 1004659200000
      },
      "content-version": "vor",
      "delay-in-days": 6416,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1984,
        4,
        9
      ]
    ]
  },
  "abstract": "<jats:p>The permeability properties of the outer membrane of the chloroplast envelope were studied. The exclusion limit for the penetration of molecules through this membrane into the intermembrane space lies between <jats:italic>M</jats:italic>\n<jats:sub>r</jats:sub> values of about 7000\u201313 000. This is the largest exclusion limit measured so far for pores of the porin type. In addition, the pore\u2010forming activity of the outer membrane of the chloroplast envelope was measured by reconstitution experiments. The pore has a single channel conductance of 0.72 nS, which is a linear function of the salt solution in the aqueous phase. The diameter of the chloroplast porin was calculated to be 2.5\u20133 nm, which is the largest diameter of all known porin pores, e.g., from mitochondria or Gram\u2010negative bacteria.</jats:p>",
  "DOI": "10.1016/0014-5793(84)80294-x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2002,
        7,
        25
      ]
    ],
    "date-time": "2002-07-25T07:59:22Z",
    "timestamp": 1027583962000
  },
  "page": "85-89",
  "source": "Crossref",
  "is-referenced-by-count": 106,
  "title": "Pore\u2010forming activity in the outer membrane of the chloroplast envelope",
  "prefix": "10.1002",
  "volume": "169",
  "author": [
    {
      "given": "U.Ingo",
      "family": "Fl\u00fcgge",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Roland",
      "family": "Benz",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2001,
        11,
        2
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.202030"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(71)90133-2"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "215",
      "volume-title": "The Intact Chloroplast",
      "author": "Heldt H.W.",
      "year": "1976"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.70.4.1032"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.60.2.286"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0065-2911(08)60208-8"
    },
    {
      "key": "e_1_2_1_8_1",
      "first-page": "123",
      "volume-title": "Transport in Biomembranes: Model Systems and Reconstitution",
      "author": "Benz R.",
      "year": "1982"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)85942-2"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1982.tb06578.x"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(82)90114-6"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(74)90174-1"
    },
    {
      "key": "e_1_2_1_13_1",
      "first-page": "153",
      "volume-title": "Biochemistry and Metabolism of Plant Lipids",
      "author": "Dorne J.",
      "year": "1982"
    },
    {
      "key": "e_1_2_1_14_1",
      "first-page": "203",
      "author": "Block M.A.",
      "year": "1983",
      "journal-title": "Sixth International Congress on Photosynthesis"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(78)90706-6"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(67)10111-0"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(70)80140-5"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(78)90269-9"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(89)90002-3"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(76)90913-X"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(79)90373-0"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(17)37790-6"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1085/jgp.77.2.121"
    },
    {
      "key": "e_1_2_1_24_1",
      "first-page": "225",
      "volume": "38",
      "author": "Renkin E.M.",
      "year": "1954",
      "journal-title": "J. Gen. Physiol."
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(75)90105-4"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(81)90336-9"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(81)90240-1"
    },
    {
      "key": "e_1_2_1_28_1",
      "author": "Benz R.",
      "year": "1984",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "e_1_2_1_29_1",
      "first-page": "315",
      "volume": "44",
      "author": "Carde J.P.",
      "year": "1982",
      "journal-title": "Biol. Cell."
    }
  ],
  "container-title": "FEBS Letters",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2884%2980294-X",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(84)80294-X",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2884%2980294-X",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        16
      ]
    ],
    "date-time": "2023-09-16T08:17:34Z",
    "timestamp": 1694852254000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1016/0014-5793%2884%2980294-X"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1984,
        4,
        9
      ]
    ]
  },
  "references-count": 28,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1984,
          4,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1016/0014-5793(84)80294-X"
  ],
  "URL": "http://dx.doi.org/10.1016/0014-5793(84)80294-X",
  "relation": {},
  "ISSN": [
    "0014-5793",
    "1873-3468"
  ],
  "subject": [],
  "container-title-short": "FEBS Letters",
  "published": {
    "date-parts": [
      [
        1984,
        4,
        9
      ]
    ]
  }
}