DOI: 10.1016/0014-5793(84)80098-8. Purification and characterization of cell wall lytic enzyme released by mating gametes of <i>Chlamydomonas reinhardtii</i>

Purification and characterization of cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii

10.1016/0014-5793(84)80098-8

Crossref journal-article

Wiley

FEBS Letters (311)

Abstract

A cell wall lytic enzyme of Chlamydomonas reinhardtii has been purified and identified as a single glycopolypeptide subunit of 62 kDa by SDS—polyacrylamide gel electrophoresis. It is released into culture medium by mating gametes as a large aggregate of subunits. The purified enzyme shows a pH optimum at about 7.5 and 35°C. Metal ion chelators and SH‐blocking agents inhibit the activity. The activity is also diminished by α2‐macroglobulin.

Bibliography

Matsuda, Y., Yamasaki, A., Tatsuaki, S., & Yamaguchi, T. (1984). Purification and characterization of cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii. FEBS Letters, 166(2), 293â297. Portico.

Authors 4

Yoshihiro Matsuda (first)
Atsuko Yamasaki (additional)
Saito Tatsuaki (additional)
Tetsuya Yamaguchi (additional)

References 23 Referenced 30

10.1007/BF00424874
{'key': 'e_1_2_1_3_1', 'first-page': '1253', 'volume': '19', 'author': 'Matsuda Y.', 'year': '1978', 'journal-title': 'Plant Cell Physiol.'} / Plant Cell Physiol. by Matsuda Y. (1978)
10.1038/265444a0
10.1083/jcb.97.2.397
10.1016/S0014-4827(77)80028-1
10.1016/0014-4827(82)90096-9
10.1111/j.1438-8677.1976.tb03562.x
10.1007/BF00387749
10.1007/BF00445732
10.1007/BF00445873
10.1093/pcp/24.2.255
{'key': 'e_1_2_1_13_1', 'first-page': '127', 'volume': '22', 'author': 'Tamaki S.', 'year': '1981', 'journal-title': 'Plant Cell Physiol.'} / Plant Cell Physiol. by Tamaki S. (1981)
10.1007/BF01053986
10.1016/S0021-9258(19)52451-6
10.1016/S0021-9258(18)61792-2
10.1126/science.6162199
10.1016/0003-2697(82)90593-0
10.1083/jcb.91.1.167
10.1016/0022-2836(75)90170-9
10.1007/BF00408711
10.1093/oxfordjournals.pcp.a076133
10.1111/j.1438-8677.1981.tb03413.x
{'key': 'e_1_2_1_24_1', 'first-page': '1093', 'volume-title': 'Methods in Chloroplast Molecular Biology', 'author': 'Gray J.C.', 'year': '1982'} / Methods in Chloroplast Molecular Biology by Gray J.C. (1982)

Dates

Type	When
Created	23 years ago (July 25, 2002, 3:59 a.m.)
Deposited	1 year, 11 months ago (Sept. 16, 2023, 3:50 p.m.)
Indexed	4 weeks ago (July 24, 2025, 7:38 a.m.)
Issued	41 years, 6 months ago (Jan. 30, 1984)
Published	41 years, 6 months ago (Jan. 30, 1984)
Published Online	23 years, 9 months ago (Nov. 14, 2001)
Published Print	41 years, 6 months ago (Jan. 30, 1984)

Funders 0

None

BibTeX

@article{Matsuda_1984, title={Purification and characterization of cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii}, volume={166}, ISSN={1873-3468}, url={http://dx.doi.org/10.1016/0014-5793(84)80098-8}, DOI={10.1016/0014-5793(84)80098-8}, number={2}, journal={FEBS Letters}, publisher={Wiley}, author={Matsuda, Yoshihiro and Yamasaki, Atsuko and Tatsuaki, Saito and Yamaguchi, Tetsuya}, year={1984}, month=jan, pages={293–297} }

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